Abstract
The efficient recycling of synaptic vesicles (SVs) during neuronal activity is central for sustaining brain function. During intense neuronal activity, the dominant mechanism of SV retrieval is activity-dependent bulk endocytosis (ADBE). Here, we describe a method to monitor ADBE in isolation from other SV endocytosis modes, via the uptake of large fluorescent fluid-phase markers in primary neuronal culture. Furthermore, we outline how to monitor ADBE using this approach across a field of neurons or in individual neurons.
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Acknowledgments
This work was supported by grants awarded by Cure Huntington’s Disease Initiative (A-11210 and A-14021; to KS and MC) and the Wellcome Trust (204954/Z/16/Z to MC).
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Cousin, M.A., Smillie, K.J. (2021). Monitoring Activity-Dependent Bulk Endocytosis in Primary Neuronal Culture Using Large Fluorescent Dextrans. In: Niedergang, F., Vitale, N., Gasman, S. (eds) Exocytosis and Endocytosis. Methods in Molecular Biology, vol 2233. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1044-2_7
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DOI: https://doi.org/10.1007/978-1-0716-1044-2_7
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