Abstract
Ca2+ regulates a variety of cellular processes that are essential to maintain cell integrity and function. Different methods have been used to study these processes by increasing intracellular Ca2+ levels. Here, we describe a protocol to initiate Ca2+-dependent membrane-related events, using laser ablation by near-infrared irradiation. This creates a rupture in the plasma membrane that allows the extracellular Ca2+ to enter the cell and thereby induce a receptor-independent Ca2+ increase. We report laser ablation protocols to study two different Ca2+-induced processes in human endothelial cells—membrane resealing and exocytosis of secretory granules called Weibel-Palade bodies (WPBs). Thus, laser ablation represents a technique that permits the analysis of different Ca2+-regulated processes at high spatiotemporal resolution in a controlled manner.
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Ashraf, A.P.K., Koerdt, S.N., Raj, N., Gerke, V. (2021). Induction of Ca2+-Dependent Exocytotic Processes by Laser Ablation of Endothelial Cells. In: Niedergang, F., Vitale, N., Gasman, S. (eds) Exocytosis and Endocytosis. Methods in Molecular Biology, vol 2233. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1044-2_19
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DOI: https://doi.org/10.1007/978-1-0716-1044-2_19
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1043-5
Online ISBN: 978-1-0716-1044-2
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