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Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated with Perilacunar/Canalicular Remodeling of the Bone Matrix

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Skeletal Development and Repair

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2230))

Abstract

Recent advances have revived interest in the concept of osteocyte perilacunar/canalicular remodeling (PLR) and have motivated efforts to identify the mechanisms regulating this process in bone in the context of normal physiology and pathological conditions. Here, we describe several methods that are evaluating morphological changes associated with PLR function of osteocytes.

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Acknowledgments

The authors gratefully acknowledge funding from NIH R01DE019284, P30AR061312, R21AR070403; NSF 1636331 and Center for Disruptive Musculoskeletal Innovation, DOD OR170044, and the Read Research Foundation (TA), NIA-1F31AG063402-01A1 (CS), and NIH P01AG039355 and R21AR054449 (SD). We acknowledge use of the UMKC Confocal Microscopy Core supported by NIH grants S10RR027668 and S10OD021665.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, University of California, San Francisco, San Francisco, CA, USA

    Neha S. Dole, Cristal S. Yee, Charles A. Schurman & Tamara Alliston

  2. UC Berkeley/UCSF Graduate Program in Bioengineering, San Francisco, CA, USA

    Charles A. Schurman & Tamara Alliston

  3. Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri Kansas City, Kansas City, MO, USA

    Sarah L. Dallas

Authors
  1. Neha S. Dole
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  2. Cristal S. Yee
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  3. Charles A. Schurman
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  4. Sarah L. Dallas
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  5. Tamara Alliston
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Corresponding author

Correspondence to Tamara Alliston .

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Editors and Affiliations

  1. Department of Orthopaedic Surgery and Cell Biology, Duke Cellular, Developmental, and Genome Laboratories, Duke University School of Medicine, Durham, USA

    Matthew J. Hilton

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Dole, N.S., Yee, C.S., Schurman, C.A., Dallas, S.L., Alliston, T. (2021). Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated with Perilacunar/Canalicular Remodeling of the Bone Matrix. In: Hilton, M.J. (eds) Skeletal Development and Repair. Methods in Molecular Biology, vol 2230. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1028-2_17

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  • DOI: https://doi.org/10.1007/978-1-0716-1028-2_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1027-5

  • Online ISBN: 978-1-0716-1028-2

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