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Gene Inactivation in Adult Long-Term Hematopoietic Stem Cells Using Inducible Mouse Models

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Mouse Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2224))

Abstract

The availability of mouse models that allow inducible long-term hematopoietic stem cell (LT-HSC)-specific gene deletion in adult mice has been limited. Therefore, analysis of gene function in adult LT-HSCs has mostly relied on models such as the interferon inducible Mx1-Cre model. Unfortunately, the Mx1-Cre strain has significant drawbacks due to lack of specificity towards the hematopoietic system, adverse effects of interferon induction on the interpretation of data, and Cre expression leakage. In this chapter, we will describe the use of other inducible models, the tamoxifen-inducible Cre-ERT and Cre-ERT2 strains, and how these mice can be used to study gene function in LT-HSC.

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Acknowledgments

This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract HHSN261200800001E. This research was supported, in part, by the Intramural Research Program of National Institutes of Health, National Cancer Institute, and Center for Cancer Research. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government.

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Authors and Affiliations

  1. Hematopoiesis and Stem Cell Biology Section, Mouse Cancer Genetics Program, Center for Cancer Research-National Cancer Institute, Frederick, MD, USA

    Kristbjorn Orri Gudmundsson

  2. Basic Research Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, MD, USA

    Kristbjorn Orri Gudmundsson

Authors
  1. Kristbjorn Orri Gudmundsson
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Correspondence to Kristbjorn Orri Gudmundsson .

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Editors and Affiliations

  1. Basic Research Laboratory, National Cancer Institute, Frederick, MD, USA

    Shree Ram Singh

  2. Department of Surgery, University of California, AntiCancer, Inc., San Diego, CA, USA

    Robert M. Hoffman

  3. Department of Biology, University of Dayton, Dayton, OH, USA

    Amit Singh

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Gudmundsson, K.O. (2021). Gene Inactivation in Adult Long-Term Hematopoietic Stem Cells Using Inducible Mouse Models. In: Singh, S.R., Hoffman, R.M., Singh, A. (eds) Mouse Genetics . Methods in Molecular Biology, vol 2224. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1008-4_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1008-4_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1007-7

  • Online ISBN: 978-1-0716-1008-4

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