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Developmental Biology of the Sea Urchin and Other Marine Invertebrates pp 31–48Cite as

Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2219))

Abstract

Pseudopotamilla occelata is a polychaete worm distributed widely in the northern part of the Pacific coast, having value as fishing bait as well as biological material for some basic research areas, including reproduction. Here we describe methods for handling the gametes and embryos of this worm, focusing on such topics as maintenance of adults, induction of oocyte maturation and fertilization, culture of embryos and larvae, microinjection into oocytes, and calcium (Ca2+) imaging.

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Acknowledgments

This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI under grant numbers 26440177 and 17K07482 for R.D.

Author information

Authors and Affiliations

  1. Miyagi University of Education, Sendai, Miyagi, Japan

    Ryusaku Deguchi

  2. Sendai Daisan High School, Sendai, Miyagi, Japan

    Takeshi Nakano

Authors
  1. Ryusaku Deguchi
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  2. Takeshi Nakano
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Corresponding author

Correspondence to Ryusaku Deguchi .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, Midwestern University, Glendale, AZ, USA

    David J. Carroll

  2. Department of Biology, University of New Mexico, Albuquerque, NM, USA

    Stephen A. Stricker

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Deguchi, R., Nakano, T. (2021). Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata. In: Carroll, D.J., Stricker, S.A. (eds) Developmental Biology of the Sea Urchin and Other Marine Invertebrates. Methods in Molecular Biology, vol 2219. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0974-3_2

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  • DOI: https://doi.org/10.1007/978-1-0716-0974-3_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0973-6

  • Online ISBN: 978-1-0716-0974-3

  • eBook Packages: Springer Protocols

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