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Transcriptomic Analysis in the Sea Anemone Nematostella vectensis

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Developmental Biology of the Sea Urchin and Other Marine Invertebrates

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2219))

Abstract

The sea anemone Nematostella vectensis is an emerging research model to study embryonic development and regeneration at the molecular and global transcriptomic level. Transcriptomics analysis is now routinely used to detect differential expression at the genome level. Here we present the latest procedures for isolating high-quality RNA required for next generation sequencing, as well as methods and resources for quantifying transcriptomic data.

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Author information

Authors and Affiliations

  1. Université Côte d’Azur, CNRS, INSERM, Institute for Research on Cancer and Aging, Nice (IRCAN), Nice, France

    Jacob F. Warner & Eric Röttinger

  2. Department of Biology and Marine Biology, University of North Carolina at Wilmington, Wilmington, NC, USA

    Jacob F. Warner

Authors
  1. Jacob F. Warner
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  2. Eric Röttinger
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Corresponding author

Correspondence to Eric Röttinger .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, Midwestern University, Glendale, AZ, USA

    David J. Carroll

  2. Department of Biology, University of New Mexico, Albuquerque, NM, USA

    Stephen A. Stricker

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Warner, J.F., Röttinger, E. (2021). Transcriptomic Analysis in the Sea Anemone Nematostella vectensis. In: Carroll, D.J., Stricker, S.A. (eds) Developmental Biology of the Sea Urchin and Other Marine Invertebrates. Methods in Molecular Biology, vol 2219. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0974-3_14

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  • DOI: https://doi.org/10.1007/978-1-0716-0974-3_14

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0973-6

  • Online ISBN: 978-1-0716-0974-3

  • eBook Packages: Springer Protocols

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