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Quantification of Tumor Vasculature by Analysis of Amount and Spatial Dispersion of Caliber-Classified Vessels

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Vascular Morphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2206))

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Abstract

This protocol focuses on the quantitative description of the angioarchitecture of experimental tumor xenografts. This semiautomatic analysis is carried out on functional vessels and microvessels acquired by confocal imaging and processed into progressively reconstructed angioarchitectures following a caliber-classification step. The protocol can be applied also to the quantification of pathological angioarchitectures other than tumor grafts as well as to the microvasculature of physiological tissue samples.

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Acknowledgments

This work was supported by Fondazione Cariplo grant no. 2016-0570 to AG and also supported by “Regione Lombardia, Progetto GenePark (#149065)”.

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Authors and Affiliations

  1. Consiglio Nazionale delle Ricerche, Institute of Neuroscience, Milano, Italy

    Marco Righi

  2. NeuroMI Milan Center for Neuroscience, University of Milano-Bicocca, Milan, Italy

    Marco Righi

  3. Unit of Experimental Oncology and Immunology, Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy

    Marco Presta & Arianna Giacomini

Authors
  1. Marco Righi
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  2. Marco Presta
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  3. Arianna Giacomini
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Corresponding author

Correspondence to Marco Righi .

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Editors and Affiliations

  1. Department of Basic Medical Sciences, Neurosciences and Sensory Organs, University of Bari Medical School, Bari, Italy

    Domenico Ribatti

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Righi, M., Presta, M., Giacomini, A. (2021). Quantification of Tumor Vasculature by Analysis of Amount and Spatial Dispersion of Caliber-Classified Vessels. In: Ribatti, D. (eds) Vascular Morphogenesis. Methods in Molecular Biology, vol 2206. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0916-3_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0916-3_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0915-6

  • Online ISBN: 978-1-0716-0916-3

  • eBook Packages: Springer Protocols

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