Abstract
All viruses have to overcome the innate immune response in order to establish infection. Methods have been developed to assay if, and how, viruses overcome these responses, and many can be directly applied to coronaviruses. Here, in vitro methods to determine how coronaviruses overcome this response are described.
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References
Kell AM, Gale M Jr (2015) RIG-I in RNA virus recognition. Virology 479-480:110–121. https://doi.org/10.1016/j.virol.2015.02.017
Chattopadhyay S, Sen GC (2017) RIG-I-like receptor-induced IRF3 mediated pathway of apoptosis (RIPA): a new antiviral pathway. Protein Cell 8(3):165–168. https://doi.org/10.1007/s13238-016-0334-x
Matthews KL, Coleman CM, van der Meer Y, Snijder EJ, Frieman MB (2014) The ORF4b-encoded accessory proteins of Middle East respiratory syndrome coronavirus and two related bat coronaviruses localize to the nucleus and inhibit innate immune signalling. J Gen Virol 95(Pt 4):874–882. https://doi.org/10.1099/vir.0.062059-0
Coleman CM, Sisk JM, Halasz G, Zhong J, Beck SE, Matthews KL, Venkataraman T, Rajagopalan S, Kyratsous CA, Frieman MB (2017) CD8+ T cells and macrophages regulate pathogenesis in a mouse model of Middle East respiratory syndrome. J Virol 91(1). https://doi.org/10.1128/JVI.01825-16
Coleman CM, Frieman MB (2015) Growth and quantification of MERS-CoV infection. Curr Protoc Microbiol 37:15E.2.1-9. https://doi.org/10.1002/9780471729259.mc15e02s37
Neil SJ, Zang T, Bieniasz PD (2008) Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu. Nature 451(7177):425–430. https://doi.org/10.1038/nature06553
Wang SM, Huang KJ, Wang CT (2014) BST2/CD317 counteracts human coronavirus 229E productive infection by tethering virions at the cell surface. Virology 449:287–296. https://doi.org/10.1016/j.virol.2013.11.030
Taylor JK, Coleman CM, Postel S, Sisk JM, Bernbaum JG, Venkataraman T, Sundberg EJ, Frieman MB (2015) Severe acute respiratory syndrome coronavirus ORF7a inhibits bone marrow stromal antigen 2 virion tethering through a novel mechanism of glycosylation interference. J Virol 89(23):11820–11833. https://doi.org/10.1128/JVI.02274-15
Scobey T, Yount BL, Sims AC, Donaldson EF, Agnihothram SS, Menachery VD, Graham RL, Swanstrom J, Bove PF, Kim JD, Grego S, Randell SH, Baric RS (2013) Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus. Proc Natl Acad Sci U S A 110(40):16157–16162. https://doi.org/10.1073/pnas.1311542110
Almazan F, DeDiego ML, Sola I, Zuniga S, Nieto-Torres JL, Marquez-Jurado S, Andres G, Enjuanes L (2013) Engineering a replication-competent, propagation-defective Middle East respiratory syndrome coronavirus as a vaccine candidate. mBio 4(5):e00650-13. https://doi.org/10.1128/mBio.00650-13
Morner A, Bjorndal A, Albert J, Kewalramani VN, Littman DR, Inoue R, Thorstensson R, Fenyo EM, Bjorling E (1999) Primary human immunodeficiency virus type 2 (HIV-2) isolates, like HIV-1 isolates, frequently use CCR5 but show promiscuity in coreceptor usage. J Virol 73(3):2343–2349
Acknowledgment
The author would like to thank the laboratory of Dr. Matthew Frieman for providing background and details for some methods.
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Coleman, C.M. (2020). Determining How Coronaviruses Overcome the Interferon and Innate Immune Response. In: Maier, H., Bickerton, E. (eds) Coronaviruses. Methods in Molecular Biology, vol 2203. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0900-2_16
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DOI: https://doi.org/10.1007/978-1-0716-0900-2_16
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