Abstract
Autophagy constitutes an essential process triggered by oxidative stress that enables cells to recycle damaged biomolecules and organelles, which is eventually traced by immunodetection with anti-ATG8. In parallel with autophagy induction, carbon metabolism in Chlamydomonas reinhardtii under abiotic stress is diverged toward lipid biosynthesis and lipid droplet accumulation, which can be analyzed by a simple thin-layer chromatography and in vivo staining with the fluorescent probe BODIPY 493/503. We show the responses in Chlamydomonas cells exposed to mercury or cadmium (0–50 μM doses), as examples of oxidative stress-mediated changes in autophagy and lipid metabolism, monitored with the procedures described in this report.
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Acknowledgments
This work was supported by Spanish MICINN grants AGL2014-53771-R and AGL2017-87591-R.
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Ortega-Villasante, C., Barón-Sola, Á., Toledo-Basantes, M., Martínez, F., Hernández, L.E. (2021). Characterization of Oxidative Lipidomics and Autophagy Induction in Chlamydomonas reinhardtii Under Abiotic Stress. In: Espada, J. (eds) Reactive Oxygen Species. Methods in Molecular Biology, vol 2202. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0896-8_6
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