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Germ Cell Tumor Cell Culture Techniques

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Testicular Germ Cell Tumors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2195))

Abstract

Optimization of cell culture protocol for a given cell line is critical for the proper conduct of in vitro experiments. Because germ cell tumors can be so heterogeneous, optimal culture conditions can vary widely between cell lines. Here, we describe our experience in routine culture and cryopreservation of germ cell tumor cell culture. Additionally, methods for measuring cell viability and proliferation validated on these lines are provided.

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Change history

  • 28 August 2021

    The chapter was inadvertently published with Acknowledgement section excluded in the article.

References

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Acknowledgements

Research funding source: CPRIT (Cancer Prevention and Research Institute of Texas) RP170152.

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Authors and Affiliations

  1. Department of Urology, University of Texas Medical Center, Dallas, TX, USA

    John T. Lafin

  2. Cancer and Blood Disease Institute, Children’s Hospital Los Angeles, Departments of Pediatrics and Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    James F. Amatruda

  3. Department of Urology, UT Southwestern Medical Center, Dallas, TX, USA

    Aditya Bagrodia

Authors
  1. John T. Lafin
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  2. James F. Amatruda
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  3. Aditya Bagrodia
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Corresponding author

Correspondence to Aditya Bagrodia .

Editor information

Editors and Affiliations

  1. Department of Urology, UT Southwestern Medical Center, Dallas, TX, USA

    Aditya Bagrodia

  2. Cancer and Blood Disease Institute, Children’s Hospital Los Angeles, Departments of Pediatrics and Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    James F. Amatruda

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Lafin, J.T., Amatruda, J.F., Bagrodia, A. (2021). Germ Cell Tumor Cell Culture Techniques. In: Bagrodia, A., Amatruda, J.F. (eds) Testicular Germ Cell Tumors. Methods in Molecular Biology, vol 2195. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0860-9_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0860-9_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0859-3

  • Online ISBN: 978-1-0716-0860-9

  • eBook Packages: Springer Protocols

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