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Testicular Germ Cell Tumors pp 125–145Cite as

Production and Analysis of Human Primordial Germ Cell–Like Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2195))

Abstract

Primordial germ cells (PGCs) are common ancestors of all germline cells. In mammals, PGCs emerge in early-stage embryos around the timing of gastrulation at or near epiblast, and specification of PGCs from their precursor cells involves multiple growth factors secreted by adjacent cells. Recent advancements in germline stem cell biology have made it possible to generate PGC-like cell culture models (PGCLCs for PGC-like cells) from human and mouse pluripotent stem cells by mimicking the embryonic growth factor environment in vitro. Here we describe a method of producing human PGCLCs from primed-pluripotency induced pluripotent stem cells (iPSCs) via temporal conversion to naive pluripotency followed by formation of embryoid bodies (EBs) using the spin-EB method.

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Acknowledgments

We thank Shiomi Yawata and Chie Owa for technical assistance during initial studies. This study was supported by NIEHS/NIH grants R01 ES023316 and R21ES024861 to TS, and by Flight Attendant Medical Research Institute (FAMRI) grant to JHH.

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Authors and Affiliations

  1. Center for Cancer Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA

    Shino Mitsunaga, Keiko Shioda, Kurt J. Isselbacher & Toshi Shioda

  2. Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel

    Jacob H. Hanna

Authors
  1. Shino Mitsunaga
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  2. Keiko Shioda
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  3. Jacob H. Hanna
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  4. Kurt J. Isselbacher
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  5. Toshi Shioda
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Corresponding author

Correspondence to Toshi Shioda .

Editor information

Editors and Affiliations

  1. Department of Urology, UT Southwestern Medical Center, Dallas, TX, USA

    Aditya Bagrodia

  2. Cancer and Blood Disease Institute, Children’s Hospital Los Angeles, Departments of Pediatrics and Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    James F. Amatruda

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Mitsunaga, S., Shioda, K., Hanna, J.H., Isselbacher, K.J., Shioda, T. (2021). Production and Analysis of Human Primordial Germ Cell–Like Cells. In: Bagrodia, A., Amatruda, J.F. (eds) Testicular Germ Cell Tumors. Methods in Molecular Biology, vol 2195. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0860-9_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0860-9_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0859-3

  • Online ISBN: 978-1-0716-0860-9

  • eBook Packages: Springer Protocols

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