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Analysis of Lipids in Ceramide-Enriched Membrane Domains

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Lipid Rafts

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2187))

Abstract

Ceramide can be generated on cell surfaces by the activity of the acid sphingomyelinase. The unique biophysical properties of ceramide result in the self-formation of small ceramide-enriched membrane domains that spontaneously fuse to large ceramide-enriched membrane macrodomains. The present chapter describes how these domains can be labeled and thereby visualized in cells. Further, the chapter provides protocols how ceramide and sphingosine can be quantified on the surface of cells and organs.

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References

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Acknowledgments

The studies and development of the protocols was supported by multiple DFG grants over a period of 20 years.

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Authors and Affiliations

  1. Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany

    Alexander Carpinteiro & Erich Gulbins

Authors
  1. Alexander Carpinteiro
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  2. Erich Gulbins
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Corresponding author

Correspondence to Erich Gulbins .

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Editors and Affiliations

  1. Department of Physiology, University of Kentucky Medical School, Lexington, KY, USA

    Erhard Bieberich

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Carpinteiro, A., Gulbins, E. (2021). Analysis of Lipids in Ceramide-Enriched Membrane Domains. In: Bieberich, E. (eds) Lipid Rafts. Methods in Molecular Biology, vol 2187. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0814-2_11

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  • DOI: https://doi.org/10.1007/978-1-0716-0814-2_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0813-5

  • Online ISBN: 978-1-0716-0814-2

  • eBook Packages: Springer Protocols

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