Abstract
Cryoprotectants are essential to prevent ice formation during tissue cryopreservation procedures. However, the control of their concentration and spatial distribution in the tissue is necessary to avoid toxicity and other damages associated with the cryopreservation procedures, especially for bulky samples such as tissues and organs. X-ray computed tomography measures the attenuation of an X-ray beam when it passes through a substance, depending on the material properties of the samples. The high electronic density of the sulfur atom of the dimethyl sulfoxide makes it an excellent cryoprotectant to be assessed by X-ray CT, and its concentration is proportional to the X-ray attenuation either at room or cryogenic temperatures. In addition, this imaging technique also allows to detect the formation of ice and eventual fractures within tissues during the cooling and warming processes. Therefore, X-ray CT technology is an excellent tool to assess and develop new cryopreservation procedures for tissues and organs.
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References
Corral A, Balcerzyk M, Parrado-Gallego A, Fernandez-Gomez I, Lamprea DR, Olmo A, Risco R (2015) Assessment of the cryoprotectant concentration inside a bulky organ for cryopreservation using X-ray computed tomography. Cryobiology 71:419–431
Knoll GF (2010) Radiation detection and measurement, 4th edn. John Wiley & Sons, New York
Seibert JA, Boone JM (2005) X-ray imaging physics for nuclear medicine technologists. Part 2: X-ray interactions and image formation. J Nucl Med Technol 33:3–18
Bischof JC, Mahr B, Choi JH, Behling M, Mewes D (2007) Use of X-ray tomography to map crystalline and amorphous phases in frozen biomaterials. Ann Biomed Eng 35:292–304
Corral A, López R, Balcerzyk M, Parrado-Gallego A, Fernández-Gómez I, Olmo A, Risco R (2018) Use of X-ray computed tomography for ice detection applied to organ cryopreservation. Biopreserv Biobank 17:119–128
Corral A, Balcerzyk M, Gallardo M, Amorim CA, Parrado-Gallego A, Risco R (2018) An optimized controlled rate slow cooling protocol for bovine ovarian tissue cryopreservation by means of X-ray computed tomography. Theriogenology 119:183–188
Gallardo M, Paulini F, Corral A, Balcerzyk M, Lucci CM, Ambroise J, Merola M, Fernandez-Maza L, Risco R, Dolmans MM (2018) Evaluation of a new freezing protocol containing 20% DMSO concentration to cryopreserve human ovarian tissue. Reprod Biomed Online 37:653–665
Corral A, Clavero M, Gallardo M, Balcerzyk M, Amorim CA, Parrado-Gallego A, Dolmans MM, Paulini F, Morris J, Risco R (2018) Ovarian tissue cryopreservation by stepped vitrification and monitored by X-ray computed tomography. Cryobiology 81:17–26
Acosta P, Corral A, Balcerzyk M, Parrado A, Risco R (2015) Fractures, ice and Me2SO concentration under the light of X-rays in a NanoCT. Cryobiology 71:554
Risco R, Corral A, Balcerzyk M, Parrado A (2015) Computer tomography for avoiding fractures, controlling ice and monitoring cryoprotectant in organ cryopreservation. Cryobiology 71:175
Corral A, Olmo A, Balcerzyk M, Regalado D, Cobos J, Risco R (2015) Monitorización mediante TAC de procesos de preservación en frío y criopreservación de material biológico. Patent ES-2529265, WO2015007928 A1, 30 Sept 2015
Acknowledgments
This work has been supported by the Junta de Andalucía, Proyectos de Investigación de Excelencia (P08-CTS-03965), and Siemens Healthcare S.L.U. (2729/0708).
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Corral, A., Olmo, A., Risco, R. (2021). Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes. In: Wolkers, W.F., Oldenhof, H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 2180. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0783-1_12
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DOI: https://doi.org/10.1007/978-1-0716-0783-1_12
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