Abstract
The amounts of active ingredients present in the natural products are always fairly low. The lab-intensive and time-consuming extraction and isolation process has been the bottleneck in exploring natural products for drug development. Extraction is the first step to separate the desired natural products from the plants, marine organisms or microbial fermentation medium. Liquid–liquid extraction method is very commonly used for the extraction of bioactive compounds from Actinobacteria. The components present in the natural extract are complex and contain a variety of natural products that require further separation and purification to obtain the active compounds. The separation depends on the physical or chemical difference of the individual compounds present in the crude extract. Chromatography, especially TLC, column chromatography, and HPLC are the main methods used to obtain pure natural products from a complex mixture. However, the percentage of purity to be obtained may vary with the different chromatographic method. Once the compound isolated in pure, the structure elucidation is rather routine work. This owes to amazing development of modern spectroscopic techniques. In this chapter different chromatographic and spectroscopic methods are described for the isolation and characterization of bioactive compounds from actinobacteria.
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References
Dehnad A, Hamedi J, Derakhshan-Khadivi F et al (2015) Green synthesis of gold nanoparticles by a metal resistant Arthrobacter nitroguajacolicus isolated from gold mine. IEEE Trans Nanobioscience 14(4):393–396
Gopikrishnan V, Radhakrishnan M, Shanmugasundaram T et al (2019) Isolation, characterization and antifouling activity of taxifolin from Streptomyces sampsonii PM33 isolated from mangrove ecosystem, South India. Nat Sci Rep 9:12975
Choma IM, Grzelak EM (2011) Bioautography detection in thin-layer chromatography. J Chromatogr A 1218(19):2684–2691
Nunez-Montero K, Lamilla C, Abanto M et al (2019) Antarctic Streptomyces fildesensis So13.3 strain as a promising source for antimicrobials discovery. Sci Rep 9(1):7488
Raghava Rao KV, Mani P, Satyanarayana B et al (2017) Purification and structural elucidation of three bioactive compounds isolated from Streptomyces coelicoflavus BC 01 and their biological activity. 3 Biotech 7(1):24
Uzair B, Menaa F, Khan BA et al (2018) Isolation, purification, structural elucidation and antimicrobial activities of kocumarin, a novel antibiotic isolated from actinobacterium Kocuria marina CMG S2 associated with the brown seaweed Pelvetia canaliculata. Microbiol Res 206:186–197
Atta HM (2015) Biochemical studies on antibiotic production from Streptomyces sp.: taxonomy, fermentation, isolation and biological properties. J Saudi Chem Soc 19(1):12–22
Shaik M, Girija Sankar G, Iswarya M et al (2017) Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10. J Genet Eng Biotechnol 15(1):87–94
Graham Solomons TW, Fryhle CB (2011) Organic chemistry, 10th edition, Wiley, p 387
Clayden J, Greeves N, Warren S, Wothers P (2001) Proton nuclear magnetic resonance. Organic chemistry, Chapter 11, Oxford University Press, 269
Lenz EM (2010) Nuclear magnetic resonance (NMR)-based drug metabolite profiling. Methods Mol Biol 708:299–319
Dass R, Grudzia ZK, Ishikawa T et al (2017) Fast 2D NMR spectroscopy for in vivo monitoring of bacterial metabolism in complex mixtures. Front Microbiol 8:1306
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Balagurunathan, R., Radhakrishnan, M., Shanmugasundaram, T., Gopikrishnan, V., Jerrine, J. (2020). Bioassay-Guided Isolation and Characterization of Metabolites from Actinobacteria. In: Protocols in Actinobacterial Research. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-0728-2_8
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DOI: https://doi.org/10.1007/978-1-0716-0728-2_8
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