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Use of Engineered Nanoparticles (ENPs) for the Study of High-Affinity IgE FcεRI Receptor Engagement and Rat Basophilic Leukemia (RBL) Cell Degranulation

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Basophils and Mast Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2163))

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Abstract

Degranulation of mast cells and basophils occurs after the cross-linking of FcεRI receptor-bound IgE by multivalent allergens, resulting in the release of a range of de novo synthesized and preformed mediators of the allergic response. β-Hexosaminidase release is usually measured as a simple readout for degranulation. Furthermore, the rat basophilic leukemia (RBL)-2H3 cell line is commonly used for measuring degranulation, monitoring β-hexosaminidase release. Here, we describe surface-engineered and modified nanoparticles with specific ligands in order to study the signaling and cellular responses of the RBL-2H3 cell line.

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References

  1. Falcone FH, Haas H, Gibbs BF (2000) The human basophil: a new appreciation of its role in immune responses. Blood 96:4028–4038

    Article  CAS  PubMed  Google Scholar 

  2. Turner H, Kinet JP (1999) Signalling through the high-affinity IgE receptor Fc epsilon RI. Nature 402:B24–B30

    Article  CAS  PubMed  Google Scholar 

  3. Sánchez-Mejorada G, Rosales C (1998) Signal transduction by immunoglobulin Fc receptors. J Leukoc Biol 63:521–533

    Article  PubMed  Google Scholar 

  4. Kinet JP (1999) The high-affinity IgE receptor (Fc epsilon RI): from physiology to pathology. Annu Rev Immunol 17:931–972

    Article  CAS  PubMed  Google Scholar 

  5. Hamawy MM, Mergenhagen SE, Siraganian RP (1995) Protein tyrosine phosphorylation as a mechanism of signalling in mast cells and basophils. Cell Signal 7:535–544

    Article  CAS  PubMed  Google Scholar 

  6. Siraganian RP (2003) Mast cell signal transduction from the high-affinity IgE receptor. Curr Opin Immunol 15:639–646

    Article  CAS  PubMed  Google Scholar 

  7. Knol EF (2006) Requirements for effective IgE cross-linking on mast cells and basophils. Mol Nutr Food Res 50:620–624

    Article  CAS  PubMed  Google Scholar 

  8. Hajkova Z, Bugajev V, Draberova E et al (2010) STIM1-directed reorganization of microtubules in activated mast cells. J Immunol 186:913–923

    Article  PubMed  Google Scholar 

  9. Jabril-Cuenod B, Zhang C, Scharenberg AM et al (2002) Syk-dependent phosphorylation of Shc. J Biol Chem 271:16268–16272

    Article  Google Scholar 

  10. Song JS, Gomez J, Stancato LF, Rivera J (1996) Association of a p95 Vav-containing signaling complex with the FcεRI γ chain in the RBL-2H3 mast cell line. Evidence for a constitutive in vivo association of Vav with Grb2, Raf-1, and ERK2 in an active complex. J Biol Chem 271:26962–26970

    Article  CAS  PubMed  Google Scholar 

  11. Barsumian EL, Isersky C, Petrino MG, Siraganian RP (1981) IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur J Immunol 11:317–323

    Article  CAS  PubMed  Google Scholar 

  12. Schwartz LB, Austen KF, Wasserman SI (1979) Immunologic release of beta-hexosaminidase and beta-glucuronidase from purified rat serosal mast cells. J Immunol 123:1445–1450

    Article  CAS  PubMed  Google Scholar 

  13. Passante E, Frankish N (2009) The RBL-2H3 cell line: its provenance and suitability as a model for the mast cell. Inflamm Res 58:737–745

    Article  CAS  PubMed  Google Scholar 

  14. Feltis BN, Elbaz A, Wright PFA et al (2015) Characterizing the inhibitory action of zinc oxide nanoparticles on allergic-type mast cell activation. Mol Immunol 66:139–146

    Article  CAS  PubMed  Google Scholar 

  15. Ryan JJ, Bateman HR, Stover A et al (2014) Fullerene nanomaterials inhibit the allergic response. J Immunol 179:665–672

    Article  Google Scholar 

  16. Kang HG, Kim S, Lee KH et al (2017) 5 nm silver nanoparticles amplify clinical features of atopic dermatitis in mice by activating mast cells. Small 13:1602363

    Article  Google Scholar 

  17. Alsaleh NB, Persaud I, Brown JM (2016) Silver nanoparticle-directed mast cell degranulation is mediated through calcium and PI3K signaling independent of the high affinity IgE receptor. PLoS One 11:e0167366

    Article  PubMed  PubMed Central  Google Scholar 

  18. Radauer-Preiml I, Andosch A, Hawranek T et al (2016) Nanoparticle-allergen interactions mediate human allergic responses: protein corona characterization and cellular responses. Part Fibre Toxicol 13:3

    Article  PubMed  PubMed Central  Google Scholar 

  19. Molina N, Martin-Serrano A, Fernandez TD et al (2018) Dendrimeric antigens for drug allergy diagnosis: a new approach for basophil activation tests. Molecules 23

    Google Scholar 

  20. Gamazo C, Gastaminza G, Ferrer M et al (2014) Nanoparticle based-immunotherapy against allergy. Immunotherapy 6:885–897

    Article  CAS  PubMed  Google Scholar 

  21. Balenga NAB, Zahedifard F, Weiss R et al (2006) Protective efficiency of dendrosomes as novel nano-sized adjuvants for DNA vaccination against birch pollen allergy. J Biotechnol 124:602–614

    Article  CAS  PubMed  Google Scholar 

  22. Gómez S, Gamazo C, San Roman B et al (2009) A novel nanoparticulate adjuvant for immunotherapy with Lolium perenne. J Immunol Methods 348:1–8

    Article  PubMed  Google Scholar 

  23. Jiang W, Kim BYS, Rutka JT, Chan WCW (2008) Nanoparticle-mediated cellular response is size-dependent. Nat Nanotechnol 3:145–150

    Article  CAS  PubMed  Google Scholar 

  24. Chatterjee S, Velicer LF, Sweeley CC (1975) Glycosphingolipid glycosyl hydrolases and glycosidases of synchronized human KB cells. J Biol Chem 250:4972–4979

    Article  CAS  PubMed  Google Scholar 

  25. Wang X, Cato P, Lin H-C et al (2013) Optimisation and use of humanised RBL NF-AT-GFP and NF-AT-DsRed reporter cell lines suitable for high-throughput scale detection of allergic sensitisation in array format and identification of the ECM-integrin interaction as critical factor. Mol Biotechnol 56:136–146

    Article  PubMed Central  Google Scholar 

  26. Sofia SJ, Premnath V, Merrill EW (1998) Poly(ethylene oxide) grafted to silicon surfaces: grafting density and protein adsorption. Macromolecules 31:5059–5070

    Article  CAS  PubMed  Google Scholar 

  27. Kamyshny A, Toledano O, Magdassi S (1999) Adsorption of hydrophobized IgG and gelatin onto phosphatidyl choline-coated silica. Colloids Surf B Biointerfaces 13:187–194

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by the Higher Ministry of Education in Saudi Arabia.

Author information

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah, Kingdom of Saudi Arabia

    Walla Alelwani

  2. Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Albaha University, Albaha, Kingdom of Saudi Arabia

    Raed A. Alharbi

  3. Merck Group, Glasgow, Scotland, UK

    Daniel Wan

  4. Institute for Parasitology, Justus-Liebig-University Giessen, Giessen, Germany

    Franco. H. Falcone

  5. Division of Molecular Therapeutics and Formulation, School of Pharmacy, University of Nottingham, Nottingham, UK

    Snow Stolnik

  6. Institute of Pharmaceutical Science, King’s College London, London, UK

    Driton Vllasaliu

Authors
  1. Walla Alelwani
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  2. Raed A. Alharbi
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  3. Daniel Wan
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  4. Driton Vllasaliu
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  5. Franco. H. Falcone
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  6. Snow Stolnik
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Corresponding author

Correspondence to Walla Alelwani .

Editor information

Editors and Affiliations

  1. Division of Experimental Allergology and Immunodermatology, Department of Human Medicine, School of Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg, Oldenburg, Germany

    Bernhard F. Gibbs

  2. Institute for Parasitology, Justus Liebig University Giessen, Biomedizinisches Forschungszentrum Seltersberg (BFS), Giessen, Germany

    Franco H. Falcone

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Alelwani, W., Alharbi, R.A., Wan, D., Vllasaliu, D., Falcone, F.H., Stolnik, S. (2020). Use of Engineered Nanoparticles (ENPs) for the Study of High-Affinity IgE FcεRI Receptor Engagement and Rat Basophilic Leukemia (RBL) Cell Degranulation. In: Gibbs, B.F., Falcone, F.H. (eds) Basophils and Mast Cells. Methods in Molecular Biology, vol 2163. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0696-4_14

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  • DOI: https://doi.org/10.1007/978-1-0716-0696-4_14

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0695-7

  • Online ISBN: 978-1-0716-0696-4

  • eBook Packages: Springer Protocols

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