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A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish

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Cardiac Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2158))

Abstract

Adult zebrafish possess an elevated cardiac regenerative capacity as compared with adult mammals. In the past two decades, zebrafish have provided a key model system for studying the cellular and molecular mechanisms of innate heart regeneration. The ease of genetic manipulation in zebrafish has enabled the establishment of a genetic ablation injury model in which over 60% of cardiomyocytes can be depleted, eliciting signs of heart failure. After this severe injury, adult zebrafish efficiently regenerate lost cardiomyocytes and reverse heart failure. In this chapter, we describe the methods for inducing genetic cardiomyocyte ablation in adult zebrafish, assessing cardiomyocyte proliferation, and histologically analyzing regeneration after injury.

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Acknowledgments

K.D.P. acknowledges support from National Institutes of Health (R35 HL150713).

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Authors and Affiliations

  1. Regeneration Next, Duke University, Durham, NC, USA

    Fei Sun, Adam R. Shoffner & Kenneth D. Poss

  2. Department of Cell Biology, Duke University School of Medicine, Durham, NC, USA

    Fei Sun, Adam R. Shoffner & Kenneth D. Poss

Authors
  1. Fei Sun
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  2. Adam R. Shoffner
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  3. Kenneth D. Poss
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Corresponding author

Correspondence to Kenneth D. Poss .

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Editors and Affiliations

  1. Department of Cell Biology, Duke University Medical Center, Durham, NC, USA

    Kenneth D. Poss

  2. Division of Cardiology, UPMC Children’s Hospital of Pittsburgh, Pittsburgh, PA, USA

    Bernhard Kühn

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Sun, F., Shoffner, A.R., Poss, K.D. (2021). A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish. In: Poss, K.D., Kühn, B. (eds) Cardiac Regeneration. Methods in Molecular Biology, vol 2158. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0668-1_7

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  • DOI: https://doi.org/10.1007/978-1-0716-0668-1_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0667-4

  • Online ISBN: 978-1-0716-0668-1

  • eBook Packages: Springer Protocols

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