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Genetic Lineage Tracing of Non-cardiomyocytes in Mice

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Cardiac Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2158))

Abstract

Genetic lineage tracing is accomplished using bi-transgenic mice, where one allele is altered to express Cre recombinase, and another allele encodes a Cre-dependent genetic reporter protein. Once Cre is activated (constitutive or in response to tamoxifen), the marker gene-expressing cells become indelibly labeled by the reporter protein. Therefore, daughter cells derived from labeled cells are permanently labeled even if the marker gene that drove Cre recombinase expression is no longer expressed in these cells. This system is commonly used to label putative progenitor cells and determine the fate of their progeny. Here, we describe the use of c-kit-based genetic lineage-tracing mouse line as an example and discuss caveats for performing these types of experiments.

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Acknowledgments

This work was supported by grants from the NIH to J.H.v.B. (HL130072) and from AHA to Z.C. (18IPA34110189).

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Authors and Affiliations

  1. Department of Medicine, Cardiovascular Division, Lillehei Heart Institute, University of Minnesota, Minneapolis, MN, USA

    Zhongming Chen & Jop H. van Berlo

  2. Stem Cell Institute and Department of Integrative Biology and Physiology, University of Minnesota, Minneapolis, MN, USA

    Jop H. van Berlo

Authors
  1. Zhongming Chen
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  2. Jop H. van Berlo
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Corresponding author

Correspondence to Jop H. van Berlo .

Editor information

Editors and Affiliations

  1. Department of Cell Biology, Duke University Medical Center, Durham, NC, USA

    Kenneth D. Poss

  2. Division of Cardiology, UPMC Children’s Hospital of Pittsburgh, Pittsburgh, PA, USA

    Bernhard Kühn

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Chen, Z., van Berlo, J.H. (2021). Genetic Lineage Tracing of Non-cardiomyocytes in Mice. In: Poss, K.D., Kühn, B. (eds) Cardiac Regeneration. Methods in Molecular Biology, vol 2158. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0668-1_24

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  • DOI: https://doi.org/10.1007/978-1-0716-0668-1_24

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0667-4

  • Online ISBN: 978-1-0716-0668-1

  • eBook Packages: Springer Protocols

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