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Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes

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Cardiac Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2158))

Abstract

Failure to regenerate myocardium after injury is a major cause of mortality and morbidity in humans. Direct differentiation of human induced pluripotent stem cells (iPSCs) into cardiomyocytes provides an invaluable resource to pursue cardiac regeneration based on cellular transplantation. Beyond the potential for clinical therapies, iPSC technology also enables the generation of cardiomyocytes to recapitulate patient-specific phenotypes, thus presenting a powerful in vitro cell-based model to understand disease pathology and guide precision medicine. Here, we describe protocols for reprogramming of human dermal fibroblasts and blood cells into iPSCs using the non-integrative Sendai virus system and for the monolayer differentiation of iPSCs to cardiomyocytes using chemically defined media.

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Acknowledgments

This work was supported by the Richard King Mellon Foundation Institute for Pediatric Research (UPMC Children’s Hospital of Pittsburgh), by a Transatlantic Network of Excellence grant by Foundation Leducq (15CVD03), Children’s Cardiomyopathy Foundation, NIH grant R01HL106302 (to B.K.), and a Career Development Award from the AHA (18CDA34110053 to L.H.). We thank Dan Roden and Kevin Bersell (Vanderbilt) for generously sharing a human iPS cell line CiPS001-13 (Vanderbilt University). We thank Ashok Srinivasan (NSABP Foundation Inc.) for his critical reading of the chapter.

Author information

Authors and Affiliations

  1. Division of Cardiology, Pediatric Institute for Heart Regeneration and Therapeutics (I-HRT), UPMC Children’s Hospital of Pittsburgh, Pittsburgh, PA, USA

    Lu Han, Jocelyn Mich-Basso & Bernhard Kühn

  2. Department of Pediatrics, University of Pittsburgh, Pittsburgh, PA, USA

    Lu Han, Jocelyn Mich-Basso & Bernhard Kühn

  3. McGowan Institute of Regenerative Medicine, Pittsburgh, PA, USA

    Bernhard Kühn

  4. Richard King Mellon Foundation Institute for Pediatric Research, Pittsburgh, PA, USA

    Lu Han, Jocelyn Mich-Basso & Bernhard Kühn

Authors
  1. Lu Han
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  2. Jocelyn Mich-Basso
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  3. Bernhard Kühn
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Corresponding author

Correspondence to Bernhard Kühn .

Editor information

Editors and Affiliations

  1. Department of Cell Biology, Duke University Medical Center, Durham, NC, USA

    Kenneth D. Poss

  2. Division of Cardiology, UPMC Children’s Hospital of Pittsburgh, Pittsburgh, PA, USA

    Bernhard Kühn

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Han, L., Mich-Basso, J., Kühn, B. (2021). Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes. In: Poss, K.D., Kühn, B. (eds) Cardiac Regeneration. Methods in Molecular Biology, vol 2158. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0668-1_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0668-1_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0667-4

  • Online ISBN: 978-1-0716-0668-1

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