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Determining the ROS and the Antioxidant Status of Leaves During Cold Acclimation

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Plant Cold Acclimation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2156))

Abstract

Cold slows down Calvin cycle activity stronger than photosynthetic electron transport, which supports production of reactive oxygen species (ROS). Even under extreme temperature conditions, most ROS are detoxified by the combined action of low-molecular weight antioxidants and antioxidant enzymes. Subsequent regeneration of the low-molecular weight antioxidants by NAD(P)H and thioredoxin/thiol–dependent pathways relaxes the electron pressure in the photosynthetic electron transport chain. In general, the chloroplast antioxidant system protects plants from severe damage of enzymes, metabolites, and cellular structures by both ROS detoxification and antioxidant recycling. Various methods have been developed to quantify ROS and antioxidant levels in photosynthetic tissues. Here, we summarize a series of exceptionally fast and easily applicable methods that show local ROS accumulation and provide information on the overall availability of reducing sugars, mainly ascorbate, and of thiols.

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Author information

Authors and Affiliations

  1. Plant Physiology, Dahlem Center of Plant Sciences, Freie Universität Berlin, Berlin, Germany

    Andras Bittner, Thomas Griebel, Jörn van Buer, Ilona Juszczak-Debosz & Margarete Baier

  2. Molecular Physiology, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany

    Ilona Juszczak-Debosz

Authors
  1. Andras Bittner
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  2. Thomas Griebel
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  3. Jörn van Buer
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  4. Ilona Juszczak-Debosz
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  5. Margarete Baier
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Corresponding author

Correspondence to Margarete Baier .

Editor information

Editors and Affiliations

  1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    Dirk K. Hincha

  2. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    Ellen Zuther

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Bittner, A., Griebel, T., van Buer, J., Juszczak-Debosz, I., Baier, M. (2020). Determining the ROS and the Antioxidant Status of Leaves During Cold Acclimation. In: Hincha, D., Zuther, E. (eds) Plant Cold Acclimation. Methods in Molecular Biology, vol 2156. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0660-5_16

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  • DOI: https://doi.org/10.1007/978-1-0716-0660-5_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0659-9

  • Online ISBN: 978-1-0716-0660-5

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