Abstract
Rad54 is a eukaryotic protein that plays an important role in homologous recombination. Rad54, a member of the Swi2/Snf2 family, binds to Holliday junctions with high specificity and promotes their branch migration in an ATP hydrolysis-dependent manner. Here we describe the methods our laboratory used to characterize the branch migration activity of Rad54. These assays are applicable for other branch migration proteins regardless of whether they have canonical helicase activity or not.
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Acknowledgments
We thank Dr. Dmitry Bugreev for his invaluable contribution to developing substrates and methods for the analysis of Rad54 branch migration activity. This work was supported by the National Cancer Institute of the National Institutes of Health (NIH) grant numbers CA188347, P30CA056036, and the Drexel-Coulter Program Award (to AVM).
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Mazina, O.M., Mazin, A.V. (2021). Branch Migration Activity of Rad54 Protein. In: Aguilera, A., Carreira, A. (eds) Homologous Recombination. Methods in Molecular Biology, vol 2153. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0644-5_11
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DOI: https://doi.org/10.1007/978-1-0716-0644-5_11
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