Abstract
Vaccination has greatly reduced the burden of human diseases caused by infectious pathogens. Systematic development of vaccine targets requires established protocols to assess immunogenicity and efficacy of such vaccine candidates. Using a leading schistosomiasis vaccine candidate, Sm-p80, as an example, we describe standardized approaches for testing the immunogenicity and efficacy of Schistosoma mansoni vaccine targets. Unlike other parasite systems in which sterile immunity is required, the goal of S. mansoni vaccine targets is overall reduction in morbidity. Methods related to the parasitological parameters described in this chapter allow for the testing of the prophylactic (reduction in adult worm burden), anti-pathology (liver and intestine egg retention), and transmission blocking (fecal egg expulsion and egg hatching rates) efficacies for the vaccine target. The RNA sequencing approaches provide basis for identification of molecular signatures predictive of desirable outcomes for schistosomiasis vaccines.
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Acknowledgments
This work was supported in part by grants from the Bill and Melinda Gates Foundation (OPP1097535) and from the NIAID/NIH SBIR (R43/R44 AI103983). The snails were supplied through a NIH-NIAID contract (HHSN2722010000051) to Biomedical Research Institute. The authors state that there are no conflicts of interest to declare. Authors thank the assistance of Dr. Govert van Dam of Leiden Medical University Center in helping us develop the CAA assay in our laboratory.
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Zhang, W., Molehin, A., Patel, P., Kim, E., Peña, A., Siddiqui, A.A. (2020). Testing of Schistosoma mansoni Vaccine Targets. In: Timson, D.J. (eds) Schistosoma mansoni. Methods in Molecular Biology, vol 2151. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0635-3_19
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DOI: https://doi.org/10.1007/978-1-0716-0635-3_19
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