Abstract
Electrophysiology is the standard method for characterizing ion channel function. Two-electrode voltage clamp is a robust and relatively simple version which can be applied to the characterization of glutamate-gated chloride channels from Schistosoma mansoni, a potential schistomicidal target. Here, the method is described in detail, with an emphasis on the investigation of S. mansoni. GluCls.
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References
Smart TG, Paoletti P (2012) Synaptic neurotransmitter-gated receptors. Cold Spring Harb Perspect Biol 4(3):a009662. https://doi.org/10.1101/cshperspect.a009662
Dufour V, Beech RN, Wever C, Dent JA, Geary TG (2013) Molecular cloning and characterization of novel glutamate-gated chloride channel subunits from Schistosoma mansoni. PLoS Pathog 9(8):e1003586. https://doi.org/10.1371/journal.ppat.1003586
Molleman A (2003) Patch clamping an introductory guide to patch clamp electrophysiology. Wiley, Hoboken, New Jersey
Guan B, Chen X, Zhang X (2013) Two-electrode voltage clamp. In: Gamper N (ed) Ion channels: methods and protocols. Methods in molecular biology 998. Humana press, Totowa, New Jersey
Rudokas MW, Varga Z, Schubert AR, Asaro AB, Silva JR (2014) The Xenopus oocyte cut-open vaseline gap voltage-clamp technique with fluorometry. J Vis Exp (85):e51040. https://doi.org/10.3791/51040
Lynagh T, Cromer BA, Dufour V, Laube B (2014) Comparative pharmacology of flatworm and roundworm glutamate-gated chloride channels: implications for potential anthelmintics. Int J Parasitol Drugs Drug Resist 4(3):244–255. https://doi.org/10.1016/j.ijpddr.2014.07.004
Smith LD, Xu WL, Varnold RL (1991) Oogenesis and oocyte isolation. Methods Cell Biol 36:45–60
Wagner CA, Friedrich B, Setiawan I, Lang F, Broer S (2000) The use of Xenopus laevis oocytes for the functional characterization of heterologously expressed membrane proteins. Cell Physiol Biochem 10(1–2):1–12. https://doi.org/10.1159/000016341
Colquhoun D (1998) Binding, gating, affinity and efficacy: the interpretation of structure-activity relationships for agonists and of the effects of mutating receptors. Br J Pharmacol 125(5):924–947
Atif M, Estrada-Mondragon A, Nguyen B, Lynch JW, Keramidas A (2017) Effects of glutamate and ivermectin on single glutamate-gated chloride channels of the parasitic nematode H. contortus. PLoS Pathog 13(10):e1006663. https://doi.org/10.1371/journal.ppat.1006663
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Lynagh, T. (2020). Characterization of Schistosoma mansoni Glutamate-Gated Chloride Channels. In: Timson, D.J. (eds) Schistosoma mansoni. Methods in Molecular Biology, vol 2151. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0635-3_14
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DOI: https://doi.org/10.1007/978-1-0716-0635-3_14
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