Localization, Extraction, and Quantification of Plant and Algal Arabinogalactan Proteins

Veenhof, Reina J.; Popper, Zoë A.

doi:10.1007/978-1-0716-0621-6_24

Reina J. Veenhof^3,4 &
Zoë A. Popper^3,4

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2149))

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Abstract

Arabinogalactan proteins are a diverse group of cell wall-associated proteoglycans. While structural and molecular genetic analyses have contributed to the emerging improved understanding of the wide-range of biological processes in which AGPs are implicated; the ability to detect, localize, and quantify them is fundamentally important. This chapter describes three methods: histological staining, radial gel diffusion, and colorimetric quantification, each of which utilize the ability of Yariv reagent to bind to AGPs.

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Acknowledgments

Quentin Coster, a visiting MSc student from the Université Catholique de Louvain, Belgium, and Tanya Slattery, an undergraduate project student in Botany and Plant Science at NUI Galway, are thanked for their technical assistance.

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Authors and Affiliations

Botany and Plant Science, School of Natural Sciences, The National University of Ireland Galway, Galway, Ireland
Reina J. Veenhof & Zoë A. Popper
Ryan Institute for Environmental, Marine and Energy Research, School of Natural Sciences, The National University of Ireland Galway, Galway, Ireland
Reina J. Veenhof & Zoë A. Popper

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Reina J. Veenhof
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Zoë A. Popper
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Correspondence to Zoë A. Popper .

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Botany and Plant Science School of Natural Sciences, Ryan Institute for Environmental Marine and Energy Research School of Natural Sciences, The National University of Ireland Galway, Galway, Ireland
Zoë A. Popper

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Veenhof, R.J., Popper, Z.A. (2020). Localization, Extraction, and Quantification of Plant and Algal Arabinogalactan Proteins. In: Popper, Z. (eds) The Plant Cell Wall. Methods in Molecular Biology, vol 2149. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0621-6_24

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DOI: https://doi.org/10.1007/978-1-0716-0621-6_24
Published: 03 July 2020
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0619-3
Online ISBN: 978-1-0716-0621-6
eBook Packages: Springer Protocols

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