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Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy

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The Plant Cell Wall

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2149))

Abstract

Attenuated total reflectance Fourier transform mid-infrared (ATR-FTIR) spectroscopy is widely applicable for the chemical analysis of biological materials, relatively inexpensive, requires only simple sample preparation, and is of comparatively high-throughput compared to traditional wet chemical or chromatographic methods. It is particularly well suited for the nondestructive analysis of dried and finely ground plant samples for the subsequent prediction of cell wall and other compositional or processing parameters using chemometric regression models. Furthermore, analysis of mid IR spectra by nonregression methods (e.g., principal component analysis) provides a straightforward approach for multivariate comparison of the effects of experimental, processing, and environmental treatments, and genotypic and temporal differences on chemical composition including changes in cell wall composition. There is thus great potential for using ATR-FTIR in the lignocellulosic biomass industry at a number of levels. Here we describe methods for cell wall sample preparation and generation of ATR-FTIR spectra, and suggest techniques for the statistical analysis and/or chemometric pattern recognition between the analyzed samples.

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Acknowledgments

This work was supported by European Regional Development Funding through the Welsh Government for BEACON Grant number 8056; the Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant on Energy Grasses & Biorefining (BBS/E/W/10963A01); and Supergen Bioenergy (EPSRC GR/S28204). Special thanks are also due to Prof. Luis Mur (IBERS, Aberystwyth University) for assistance with the Blumeria graminis/Brachypodium distachyon infection experiment which is used in this chapter to illustrate PCA .

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Authors and Affiliations

  1. Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK

    Ricardo M. F. da Costa, William Barrett, José Carli & Gordon G. Allison

  2. Faculty of Sciences and Technology, Department of Life Sciences, Centre for Functional Ecology, University of Coimbra, Coimbra, Portugal

    Ricardo M. F. da Costa

Authors
  1. Ricardo M. F. da Costa
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  2. William Barrett
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  3. José Carli
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  4. Gordon G. Allison
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Corresponding author

Correspondence to Gordon G. Allison .

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Editors and Affiliations

  1. Botany and Plant Science School of Natural Sciences, Ryan Institute for Environmental Marine and Energy Research School of Natural Sciences, The National University of Ireland Galway, Galway, Ireland

    Zoë A. Popper

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da Costa, R.M.F., Barrett, W., Carli, J., Allison, G.G. (2020). Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy. In: Popper, Z. (eds) The Plant Cell Wall. Methods in Molecular Biology, vol 2149. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0621-6_16

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  • DOI: https://doi.org/10.1007/978-1-0716-0621-6_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0619-3

  • Online ISBN: 978-1-0716-0621-6

  • eBook Packages: Springer Protocols

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