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CRISPR-Cas Methods pp 1–23Cite as

Wide Horizons of CRISPR-Cas-Derived Technologies for Basic Biology, Agriculture, and Medicine

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Abstract

The discovery and subsequent repurposing of the CRISPR (clustered regularly interspaced short palindromic repeats) and Cas proteins (CRISPR-associated proteins) has revolutionized our ability to manipulate DNA and RNA sequences in vitro, ex vivo, and in vivo. In this introductory chapter, we present a brief overview of basics of CRISPR-Cas-mediated genome editing and different orthologues and engineered versions of Cas protein with altered specificity and expanded targetability. More importantly, we comprehensively portray the advances made by developing diverse CRISPR-Cas-based genome modification tools and their application in basic biology, agriculture, and medicine.

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Authors and Affiliations

  1. Department of Crop Improvement Division , National Rice Research Institute, Cuttack, Odisha, India

    Kutubuddin A. Molla

  2. Crop Improvement Division, National Rice Research Institute, Cuttack, Odisha, India

    Subhasis Karmakar

  3. Institute of Biotechnology and Genetic Engineering (IBGE), Bangbandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

    M. Tofazzal Islam

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  1. Kutubuddin A. Molla
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  2. Subhasis Karmakar
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  3. M. Tofazzal Islam
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Correspondence to Kutubuddin A. Molla .

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Editors and Affiliations

  1. Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

    M. Tofazzal Islam

  2. Department of Aquatic and Crop Resource Development, National Research Council of Canada, SASKATOON, SK, Canada

    Pankaj K. Bhowmik

  3. Crop Improvement Division, ICAR-National Rice Research Institute, Cuttack, Odisha, India

    Kutubuddin A. Molla

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Molla, K.A., Karmakar, S., Islam, M.T. (2020). Wide Horizons of CRISPR-Cas-Derived Technologies for Basic Biology, Agriculture, and Medicine. In: Islam, M.T., Bhowmik, P.K., Molla, K.A. (eds) CRISPR-Cas Methods . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0616-2_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0616-2_1

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