Abstract
Detection of Zika virus (ZIKV) in the central nervous system (CNS) is a critical step when studying the pathogenesis of the infection in animal models. Both viral load determination and immunohistochemistry (IHC) staining are useful methods to quantitatively and qualitatively characterize viral infections in target tissues. Here, we describe viral RNA load determination by droplet digital PCR as well as protein detection by polymer-based IHC as effective techniques to quantify and localize ZIKV in the CNS of mice.
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Acknowledgments
This work was supported by a Foundation Grant from the Canadian Institutes of Health Research (grant no. 148361 to G.B.). G.B. is the holder of the Canada research chair on emerging viruses and antiviral resistance. We would like to thank Maude Bordeleau for revising the protocol and proofreading this manuscript.
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Enlow, W., Piret, J., Boivin, G. (2020). Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice. In: Kobinger, G., Racine, T. (eds) Zika Virus. Methods in Molecular Biology, vol 2142. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0581-3_4
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DOI: https://doi.org/10.1007/978-1-0716-0581-3_4
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