Abstract
Group A Streptococcus (GAS) often exists as an asymptomatic colonizer of the upper respiratory tract in humans. Unsurprisingly, a high proportion of symptomatic infections caused by GAS include pharyngitis. While not usually life-threatening, these infections cause significant morbidity and economic burden/loss of productivity, and can have downstream life-threatening autoimmune consequences. Modeling asymptomatic colonization in animals is, therefore, a useful tool to dissect host–bacteria interactions and to evaluate efficacy of vaccines aimed at reducing the burden of carriage. Here we describe a mouse model of nasopharyngeal colonization using nasal challenge of susceptible mice and the evaluation of subsequent bacterial burden.
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Acknowledgments
This work was supported by the Health Research Council (HRC) New Zealand and the Maurice Wilkins Centre for Molecular Biodiscovery. J.M.S.L. is a Heart Foundation NZ research fellow.
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Khemlani, A.H.J., Proft, T., Loh, J.M.S. (2020). A Mouse Nasopharyngeal Colonization Model for Group A Streptococcus. In: Proft, T., Loh, J. (eds) Group A Streptococcus. Methods in Molecular Biology, vol 2136. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0467-0_23
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DOI: https://doi.org/10.1007/978-1-0716-0467-0_23
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Online ISBN: 978-1-0716-0467-0
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