Abstract
Metabolites like melatonin are essential in determining circadian phase. In the recent years, comprehensive metabolome analyses have unveiled entire panels of small biomolecules fluctuating in a circadian fashion, thus enabling a more precise determination of inner time and understanding of how circadian clock operates at the molecular level. Emerging analytical techniques allowing for the determination of exhaled metabolites in breath show promise to gain further insights noninvasively and in vivo into circadian metabolism.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Sharma M, Palacios-Bois J, Schwartz G, Iskandar H, Thakur M, Quirion R, Nair NPV (1989) Circadian rhythms of melatonin and cortisol in aging. Biol Psychiatry 25(3):305–319. https://doi.org/10.1016/0006-3223(89)90178-9
Dallmann R, Viola AU, Tarokh L, Cajochen C, Brown SA (2012) The human circadian metabolome. Proc Natl Acad Sci U S A 109(7):2625–2629. https://doi.org/10.1073/pnas.1114410109
Davies SK, Ang JE, Revell VL, Holmes B, Mann A, Robertson FP, Cui N, Middleton B, Ackermann K, Kayser M, Thumser AE, Raynaud FI, Skene DJ (2014) Effect of sleep deprivation on the human metabolome. Proc Natl Acad Sci U S A 111(29):10761–10766
Kasukawa T, Sugimoto M, Hida A, Minami Y, Mori M, Honma S, Honma K, Mishima K, Soga T, Ueda HR (2012) Human blood metabolite timetable indicates internal body time. Proc Natl Acad Sci U S A 109(37):15036–15041. https://doi.org/10.1073/pnas.1207768109
Minami Y, Kasukawa T, Kakazu Y, Iigo M, Sugimoto M, Ikeda S, Yasui A, van der Horst GTJ, Soga T, Ueda HR (2009) Measurement of internal body time by blood metabolomics. Proc Natl Acad Sci U S A 106(24):9890–9895. https://doi.org/10.1073/pnas.0900617106
Jens H et al (2009) On-line breath analysis with PTR-TOF. J Breath Res 3(2):027004
Španěl P, Smith D (2013) Chapter 4 - Recent SIFT-MS Studies of Volatile Compounds in Physiology, Medicine and Cell Biology. In: Amann A, Smith D (eds) Volatile Biomarkers. Elsevier, Boston, p 48–76
Martinez-Lozano Sinues P, Zenobi R, Kohler M (2013) Analysis of the exhalome: a diagnostic tool of the future. Chest 144(3):746–749. https://doi.org/10.1378/chest.13-1106
Zhu JJ, Bean HD, Jimenez-Diaz J, Hill JE (2013) Secondary electrospray ionization-mass spectrometry (SESI-MS) breathprinting of multiple bacterial lung pathogens, a mouse model study. J Appl Physiol 114(11):1544–1549. https://doi.org/10.1152/japplphysiol.00099.2013
Bean HD, Zhu J, Hill JE (2011) Characterizing bacterial volatiles using secondary electrospray ionization mass spectrometry (SESI-MS). J Vis Exp 52:e2664. https://doi.org/10.3791/2664
Dillon LA, Stone VN, Croasdell LA, Fielden PR, Goddard NJ, Paul Thomas CL (2010) Optimisation of secondary electrospray ionisation (SESI) for the trace determination of gas-phase volatile organic compounds. Analyst 135(2):306–314
Zhu J, Bean HD, Kuo YM, Hill JE (2010) Fast detection of volatile organic compounds from bacterial cultures by secondary electrospray ionization-mass spectrometry. J Clin Microbiol 48(12):4426–4431. https://doi.org/10.1128/JCM.00392-10
Martinez-Lozano Sinues P, Tarokh L, Li X, Kohler M, Brown SA, Zenobi R, Dallmann R (2014) Circadian variation of the human metabolome captured by real-time breath analysis. PLoS One 9(12):e114422. https://doi.org/10.1371/journal.pone.0114422
Martinez-Lozano Sinues P, Fernandez de la Mora J (2015) Method to analyze and classify persons and organisms based on odor patterns from released vapors; US Patent No: US9121844 B1
Martinez-Lozano Sinues P, Fernandez de la Mora J (2010) Method for detecting volatile species of high molecular weight; US Patent No: US 20100264304 A1
López-Herrera J, Barrero A, Boucard A, Loscertales I, Márquez M (2004) An experimental study of the electrospraying of water in air at atmospheric pressure. J Am Soc Mass Spectrom 15(2):253–259. https://doi.org/10.1016/j.jasms.2003.10.018
Gaugg MT, Garcia Gomez D, Barrios Collado C, Vidal de Miguel G, Kohler M, Zenobi R, Martinez-Lozano Sinues P (2016) Expanding metabolite coverage of real-time breath analysis by coupling a universal secondary electrospray ionization source and high resolution mass spectrometry—a pilot study on tobacco smokers. J Breath Res 10(1):016010
Keller BO, Sui J, Young AB, Whittal RM (2008) Interferences and contaminants encountered in modern mass spectrometry. Anal Chim Acta 627(1):71–81. https://doi.org/10.1016/j.aca.2008.04.043
García-Gómez D, Gaisl T, Bregy L, Cremonesi A, Sinues PM-L, Kohler M, Zenobi R (2016) Real-time quantification of amino acids in the Exhalome by secondary electrospray ionization–mass spectrometry: a proof-of-principle study. Clin Chem 62(9):1230–1237. https://doi.org/10.1373/clinchem.2016.256909
García-Gómez D, Martínez-Lozano Sinues P, Barrios-Collado C, Vidal-De-Miguel G, Gaugg M, Zenobi R (2015) Identification of 2-alkenals, 4-hydroxy-2-alkenals, and 4-hydroxy-2,6-alkadienals in exhaled breath condensate by UHPLC-HRMS and in breath by real-time HRMS. Anal Chem 87(5):3087–3093. https://doi.org/10.1021/ac504796p
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Brown, S.A., Sinues, P. (2021). Circadian Metabolomics from Breath. In: Brown, S.A. (eds) Circadian Clocks. Methods in Molecular Biology, vol 2130. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0381-9_11
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0381-9_11
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0380-2
Online ISBN: 978-1-0716-0381-9
eBook Packages: Springer Protocols