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CRISPR/Cas9-Based Gene Editing in Soybean

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Legume Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2107))

Abstract

CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR associated Cas9)-based gene editing is a robust tool for functional genomics research and breeding programs in various crops. In soybean, a number of laboratories have obtained mutants by CRISPR/Cas9 system; however, there has been not yet a detailed method for the CRISPR/Cas9-based gene editing in soybean. Here, we describe the procedures for constructing the CRISPR/Cas9 plasmid suitable for soybean gene editing and the modified protocols for Agrobacterium-mediated soybean transformation and regeneration from cotyledonary node explants containing the Cas9/sgRNA (single guide RNA) transgenes.

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Acknowledgments

The authors are thankful to Professor Yaoguang Liu from South China Agricultural University for providing the vectors pYLsgRNA-AtU3d/LacZ, pYLsgRNA-AtU3b, pYLsgRNA-AtU6-1, pYLsgRNA-AtU6-29, and pYLCRISPR/Cas9P35S-B.

Author information

Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China

    Aili Bao & Dong Cao

  2. Institute of Research and Development, Duy Tan University, Da Nang, Vietnam

    Lam-Son Phan Tran

  3. Stress Adaptation Research Unit, RIKEN Center for Sustainable Resource Science, Yokohama, Japan

    Lam-Son Phan Tran

Authors
  1. Aili Bao
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  2. Lam-Son Phan Tran
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  3. Dong Cao
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Corresponding authors

Correspondence to Lam-Son Phan Tran or Dong Cao .

Editor information

Editors and Affiliations

  1. School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi, India

    Mukesh Jain

  2. Department of Life Sciences, School of Natural Sciences, Shiv Nadar University, Gautam Buddha Nagar, Uttar Pradesh, India

    Rohini Garg

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Bao, A., Tran, LS.P., Cao, D. (2020). CRISPR/Cas9-Based Gene Editing in Soybean. In: Jain, M., Garg, R. (eds) Legume Genomics. Methods in Molecular Biology, vol 2107. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0235-5_19

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  • DOI: https://doi.org/10.1007/978-1-0716-0235-5_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0234-8

  • Online ISBN: 978-1-0716-0235-5

  • eBook Packages: Springer Protocols

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