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NMR of Immobilized Enzymes

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Immobilization of Enzymes and Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2100))

Abstract

Solid-state NMR has become the method of choice for the assessment of protein structure for insoluble objects lacking long-range order. In this context, it is apparent that solid-state NMR is also perfectly poised toward the characterization of immobilized proteins. For these systems, it is possible to understand at the atomic level which perturbations, if any, are occurring as a result of the functionalization. Here we describe how it is possible to accomplish the NMR characterization of enzymes that have been immobilized through different approaches, and we introduce the reader to the choice of the experimental strategy that can be useful in different cases. An outlook on the level of information that can be attained is also given, in view of recent methodological advancements.

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Acknowledgments

Dr. Venita Decker and Dr. Sebastian Wegner (Bruker Biospin), and Dr. Yusuke Nishiyiama (JEOL) are acknowledged for providing the instructions for operating low-diameter rotors.

Author information

Authors and Affiliations

  1. Magnetic Resonance Center (CERM), University of Florence and Consorzio Interuniversitario, Risonanze Magnetiche di Metallo Proteine (CIRMMP), Sesto Fiorentino, Italy

    Linda Cerofolini, Enrico Ravera, Marco Fragai & Claudio Luchinat

  2. Department of Chemistry, University of Florence, Sesto Fiorentino, Italy

    Enrico Ravera, Marco Fragai & Claudio Luchinat

Authors
  1. Linda Cerofolini
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  2. Enrico Ravera
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  3. Marco Fragai
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  4. Claudio Luchinat
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Corresponding author

Correspondence to Enrico Ravera .

Editor information

Editors and Affiliations

  1. CSIC Campus Cantoblanco, Instituto de Catalisis, Madrid, Spain

    Jose M. Guisan

  2. Institute of Biotech and Biochemical Engineering, Graz University of Technology, Graz, Austria

    Juan M. Bolivar

  3. CIC biomaGUNE, Donostia-San Sebastian, Spain

    Fernando López-Gallego

  4. Campus UAM-CSIC, Institute of Catatalysis, Madrid, Spain

    Javier Rocha-Martín

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Cerofolini, L., Ravera, E., Fragai, M., Luchinat, C. (2020). NMR of Immobilized Enzymes. In: Guisan, J., Bolivar, J., López-Gallego, F., Rocha-Martín, J. (eds) Immobilization of Enzymes and Cells. Methods in Molecular Biology, vol 2100. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0215-7_24

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  • DOI: https://doi.org/10.1007/978-1-0716-0215-7_24

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0214-0

  • Online ISBN: 978-1-0716-0215-7

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