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A New Perspective on Cryotherapy: Pathogen Elimination Using Plant Shoot Apical Meristem via Cryogenic Techniques

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Plant Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2094))

Abstract

Plant pathogens cause different diseases on crops and industrial plant species that result in economic losses. Pathogen-free plant material has usually been obtained by traditional procedures such as meristem culture, thermotherapy, and chemotherapy. However, there are many limitations of these procedures such as mechanical challenges of meristem excision and low regeneration rate, low resistance to high temperatures, phytotoxicity, and mutagenic effects of the chemicals used in the procedures. Cryotherapy is a newly developed biotechnological tool that has been very effective in virus elimination from economically important plant species. This tool has overcome the abovementioned limitations. This chapter aims to highlight the importance of the cryogenic procedures (vitrification, encapsulation-vitrification, droplet vitrification, two-step freezing, dehydration, encapsulation-dehydration) in order to generate virus-free germplasm.

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Authors and Affiliations

  1. Molecular Biology and Genetics Department, Faculty of Science, Mugla Sitki Kocman University, Mugla, Turkey

    Ergun Kaya, Selin Galatali, Sevinc Guldag & Onur Celik

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  1. Ergun Kaya
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  2. Selin Galatali
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  3. Sevinc Guldag
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  4. Onur Celik
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Correspondence to Ergun Kaya .

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Editors and Affiliations

  1. Department of Life and Environmental Sciences, College of Natural and Health Sciences, Zayed University, Abu Dhabi, United Arab Emirates

    Muhammad Naseem

  2. Department of Bioinformatics, University of Würzburg, Würzburg, Germany

    Thomas Dandekar

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Kaya, E., Galatali, S., Guldag, S., Celik, O. (2020). A New Perspective on Cryotherapy: Pathogen Elimination Using Plant Shoot Apical Meristem via Cryogenic Techniques. In: Naseem, M., Dandekar, T. (eds) Plant Stem Cells. Methods in Molecular Biology, vol 2094. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0183-9_15

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  • DOI: https://doi.org/10.1007/978-1-0716-0183-9_15

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  • Publisher Name: Humana, New York, NY

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