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Chromatin Analysis of Metabolic Gene Clusters in Plants

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Plant Epigenetics and Epigenomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2093))

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Abstract

Plant metabolic gene clusters consist of neighboring genes that are involved in the biosynthesis of secondary or specialized metabolites. The genes within clusters are typically co-regulated, share a common set of chromatin marks, and code for the biosynthesis enzymes of a single metabolic pathway. Here, we describe three essential protocols for the basic analysis of metabolic gene clusters at transcription, histone modification, and metabolite level. The protocols are specified to clusters in the Arabidopsis thaliana genome and are transferable to other plant species.

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Acknowledgments

This work was supported by startup funding from the Southern University of Science and Technology and Shenzhen municipal government (ACH) and the Royal Society University Research Fellowship (UF160138) (HWN).

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Authors and Affiliations

  1. Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen, China

    Ancheng C. Huang

  2. The Milner Centre for Evolution, University of Bath, Bath, UK

    Hans-Wilhelm Nützmann

Authors
  1. Ancheng C. Huang
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  2. Hans-Wilhelm Nützmann
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Corresponding authors

Correspondence to Ancheng C. Huang or Hans-Wilhelm Nützmann .

Editor information

Editors and Affiliations

  1. Plant and AgriBiosciences Research Centre, Ryan Institute, National University of Ireland Galway (NUI Galway), Galway, Ireland

    Charles Spillane

  2. Plant and AgriBiosciences Research Centre, Ryan Institute, National University of Ireland Galway (NUI Galway), Galway, Ireland

    Peter McKeown

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Huang, A.C., Nützmann, HW. (2020). Chromatin Analysis of Metabolic Gene Clusters in Plants. In: Spillane, C., McKeown, P. (eds) Plant Epigenetics and Epigenomics . Methods in Molecular Biology, vol 2093. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0179-2_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0179-2_10

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0178-5

  • Online ISBN: 978-1-0716-0179-2

  • eBook Packages: Springer Protocols

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