Abstract
Electrochemical biosensors have shown great promise as useful point-of-care tests since they operate on electronic circuits which can be miniaturized and whose readout process can be easily automated. Here, we describe a method for the electrochemical sensing of antibodies directed against double-stranded DNA (α-dsDNA), which are often present at higher-than-normal levels in the sera of autoimmune disease patients. The method can be easily implemented in any lab and requires little investment in equipment, namely a potentiostat. An artificial reference serum sample containing known amounts of spiked-in α-dsDNA antibodies enables reporting results in absolute scale rather than titer. Once electrodes are modified with DNA and the calibration curves are made (i.e., after the biosensor construction phase), individual measurements in test samples can be obtained in as low as 35 min.
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Acknowledgments
J.P.T. is a member of the National System of Researchers (ANII, Uruguay) and the Program for the Development of Basic Science (PEDECIBA, Uruguay). P.F. obtained funding for this work from the Agencia Nacional de Innovación e Investigación (ANII, Uruguay), grant number [INI_X_2012_1_4293].
Author contributions: J.L. envisioned the project. J.P.T. and P.F. developed the method. P.F. made most of the experimental validation with the help of G.B.P.F. and J.P.T. wrote the first version of the manuscript. All authors corrected the manuscript and approved its final version.
Competing interests: The authors declare no conflicts of interests.
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Fagúndez, P., Brañas, G., Laíz, J., Tosar, J.P. (2020). Electrochemical Detection of dsDNA-Specific Antibodies. In: Astakhova, K., Bukhari, S. (eds) Nucleic Acid Detection and Structural Investigations. Methods in Molecular Biology, vol 2063. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0138-9_7
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DOI: https://doi.org/10.1007/978-1-0716-0138-9_7
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