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Behavioral Responses in Rats Submitted to Chronic Administration of Branched-Chain Amino Acids

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JIMD Reports - Case and Research Reports, Volume 13

Part of the book series: JIMD Reports ((JIMD,volume 13))

Abstract

Maple syrup urine disease (MSUD) is an inborn metabolism error caused by a deficiency of branched-chain α-keto acid dehydrogenase complex activity. This blockage leads to an accumulation of the branched-chain amino acids (BCAA) leucine, isoleucine, and valine, as well as their corresponding α-keto and α-hydroxy acids. Previous reports suggest that MSUD patients are at high risk for chronic neuropsychiatric problems. Therefore, in this study, we assessed variables that suggest depressive-like symptoms (anhedonia as measured by sucrose intake, immobility during the forced swimming test and body and adrenal gland weight) in rats submitted to chronic administration of BCAA during development. Furthermore, we determined if these parameters were sensitive to imipramine and N-acetylcysteine/deferoxamine (NAC/DFX). Our results demonstrated that animals subjected to chronic administration of branched-chain amino acids showed a decrease in sucrose intake without significant changes in body weight. We also observed an increase in adrenal gland weight and immobility time during the forced swimming test. However, treatment with imipramine and NAC/DFX reversed these changes in the behavioral tasks. In conclusion, this study demonstrates a link between MSUD and depression in rats. Moreover, this investigation reveals that the antidepressant action of NAC/DFX and imipramine might be associated with their capability to maintain pro-/anti-oxidative homeostasis.

Competing interests: None declared

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References

  • Araújo P, Wassermann GF, Tallini K et al (2001) Reduction of large neutral amino acid levels in plasma and brain of hyperleucinemic rats. Neurochem Int 38:529–537

    Article  PubMed  Google Scholar 

  • Arent CO, Réus GZ, Abelaira HM et al (2012) Synergist effects of n-acetylcysteine and deferoxamine treatment on behavioral and oxidative parameters induced by chronic mild stress in rats. Neurochem Int 61:1072–80

    Article  CAS  PubMed  Google Scholar 

  • Baker DA, Xi ZX, Shen H, Swanson CJ, Kalivas PW (2002) The origin and neuronal function of in vivo nonsynaptic glutamate. J Neurosci 22:9134–9141

    CAS  PubMed  Google Scholar 

  • Banjac A, Perisic T, Sato H et al (2008) The cystine/cysteine cycle: a redox cycle regulating susceptibility versus resistance to cell death. Oncogene 27:1618–1628

    Article  CAS  PubMed  Google Scholar 

  • Barschak AG, Sitta A, Deon M et al (2006) Evidence that oxidative stress in increased in plasma from pacients with maple syrup urine disease. Metab Brain Dis 21:279–286

    Article  CAS  PubMed  Google Scholar 

  • Berk M, Malhi GS, Gray LJ, Dean OM (2013) The promise of N-acetylcysteine in neuropsychiatry. Trends Pharmacol Sci 34:167–177

    Article  CAS  PubMed  Google Scholar 

  • Bridi R, Araldi J, Sgarbi MB et al (2003) Induction of oxidative stress in rat brain by the metabolites accumulating in maple syrup urine disease. Int J Dev Neurosci 21:327–332

    Article  CAS  PubMed  Google Scholar 

  • Bridi R, Fontella FU, Pulrolnik V et al (2006) A chemically-induced acute model of maple syrup urine disease in rats for neurochemical studies. J Neurosci Methods 155:224–230

    Article  CAS  PubMed  Google Scholar 

  • Bridi R, Latini A, Braum CA et al (2005) Evaluation of the mechanisms involved in leucine induced oxidative damage in cerebral cortex of young rats. Free Radic Res 39:71–79

    Article  CAS  PubMed  Google Scholar 

  • Cavalleri F, Berardi A, Burlina AB, Ferrari F, Mavilla L (2002) Diffusion-weighted MRI of maple syrup urine disease encephalopathy. Neuroradiology 44:499–502

    Article  CAS  PubMed  Google Scholar 

  • Chuang DT, Shih VE (2001) Maple syrup urine disease (branchedchain ketoaciduria). In: Scriver CR, Beaudet AL, Sly WS, Valle D (eds) The metabolic and molecular bases of inherited disease. McGraw-Hill, New York, pp 1971–2005

    Google Scholar 

  • Danner DJ, Elsas LJ (1989) Disorders of branched chain amino acid and keto acid metabolism. In: Scriver CR, Beaudet AL, Sly WS, Valle D (eds) The metabolic basis of inherited disease. McGraw-Hill, New York, pp 671–692

    Google Scholar 

  • DellaGioia N, Hannestad J (2010) A critical review of human endotoxin administration as an experimental paradigm of depression. Neurosci Biobehav Rev 34:130–143

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Dimopoulos N, Piperi C, Psarra V, Lea RW, Kalofoutis A (2008) Increased plasma levels of 8-iso-PGF2alpha and IL-6 in an elderly population with depression. Psychiatry Res 161:59–66

    Article  CAS  PubMed  Google Scholar 

  • Di-Pietro PB, Dias ML, Scaini G et al (2008) Inhibition of brain creatine kinase activity after renal ischemia is attenuated by N-acetylcysteine and deferoxamine administration. Neurosci Lett 434:139–143

    Article  CAS  PubMed  Google Scholar 

  • Dodd PR, Williams SH, Gundlach AL et al (1992) Glutamate and gamma-aminobutyric acid neurotransmitter systems in the acute phase of maple syrup urine disease and citrullinemia encephalopathies in newborn calves. J Neurochem 59:582–590

    Article  CAS  PubMed  Google Scholar 

  • Ferreira FR, Biojone C, Joca SRL, Guimarães FS (2008) Antidepressant-like effects of N-acetyl-L-cysteine in rats. Behav Pharmacol 19:747–50

    Article  CAS  PubMed  Google Scholar 

  • Fontella FU, Gassen E, Pulrolnik V et al (2002) Stimulation of lipid peroxidation in vitro in rat brain by the metabolites accumulating in maple syrup urine disease. Metab Brain Dis 17:47–54

    Article  CAS  PubMed  Google Scholar 

  • Forlenza MJ, Miller GE (2006) Increased serum levels of 8-hydroxy-2’-deoxyguanosine in clinical depression. Psychosom Med 68:1–7

    Article  CAS  PubMed  Google Scholar 

  • Gamaro GD, Manoli LP, Torres IL, Silveira R, Dalmaz C (2003) Effects of chronic variate stress on feeding behavior and on monoamine levels in different rat brain structures. Neurochem Int 42:107–114

    Article  CAS  PubMed  Google Scholar 

  • Gardner A, Boles RG (2011) Beyond the serotonin hypothesis: mitochondria, inflammation and neurodegeneration in major depression and affective spectrum disorders. Prog Neuro Psychopharmacol Biol Psychiatry 35:730–743

    Article  CAS  Google Scholar 

  • Gere-Paszti E, Jakus J (2009) The effect of N-acetylcysteine on amphetamine-mediated dopamine release in rat brain striatal slices by ion-pair reversed-phase high performance liquid chromatography. Biomed Chromatogr 23:658–664

    Article  CAS  PubMed  Google Scholar 

  • Gilbert KR, Aizenman E, Reynolds IJ (1991) Oxidized glutathione modulates N-methyl-D-aspartate- and depolarization-induced increases in intracellularCa2+ in cultured rat forebrain neurons. Neurosci Lett 133:11–14

    Article  CAS  PubMed  Google Scholar 

  • Ha JS, Kim TK, Eun BL et al (2004) Maple syrup urine disease encephalopathy: a follow-up study in the acute stage using diffusion-weighted MRI. Pediatr Radiol 34:163–166

    Article  PubMed  Google Scholar 

  • Howell RK, Lee M (1963) Influence of a-keto acids on the respiration of brain in vitro. Proc Soc Exp Biol Med 113:660–663

    Article  CAS  PubMed  Google Scholar 

  • Hutson SM, Lieth E, LaNoue KF (2001) Function of leucine in excitatory neurotransmitter metabolism in the central nervous system. J Nutr 131:846S–850S

    CAS  PubMed  Google Scholar 

  • Iyer SS, Jones DP, Brigham KL, Rojas M (2009) Oxidation of plasma cysteine/cystine redox state in endotoxin-induced lung injury. Am J Respir Cell Mol Biol 40:90–98

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Janáky R, Dohovics R, Saransaari P, Oja SS (2007) Modulation of [3H]dopamine release by glutathione in mouse striatal slices. Neurochem Res 32:1357–1364

    Article  PubMed  Google Scholar 

  • Jou SH, Chiu NY, Liu CS (2009) Mitochondrial dysfunction and psychiatric disorders. Chang Gung Med J 32:370–379

    PubMed  Google Scholar 

  • Jouvet P, Rustin P, Taylor DL et al (2000) Branched chain amino acids induce apoptosis in neural cells without mitochondrial membrane depolarization or cytochrome c release: implications for neurological impairment associated with maple syrup urine disease. Mol Biol Cell 11:1919–1932

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Katz RJ, Roth KA, Carroll BJ (1981a) Acute and chronic stress effects on open field activity in the rat: implications for a model of depression. Neurosci Biobehav Rev 5:247–251

    Article  CAS  PubMed  Google Scholar 

  • Katz RJ, Roth KA, Carroll BJ (1981b) Animal models and human depressive disorders. Neurosci Biobehav Rev 5:231–246

    Article  CAS  PubMed  Google Scholar 

  • Khanzode SD, Dakhale GN, Khanzode SS, Saoji A, Palasodkar R (2003) Oxidative damage and major depression: the potential antioxidant action of selective serotonin re-uptake inhibitors. Redox Rep 8:365–370

    Article  CAS  PubMed  Google Scholar 

  • Krishnan V, Nestler EJ (2008) The molecular neurobiology of depression. Nature 455:894–902

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kumar A, Garg R (2009) Possible role of trazodone and imipramine in sleep deprivation-induced anxiety-like behavior and oxidative damage in mice. Methods Find Exp Clin Pharmacol 31:383–387

    CAS  PubMed  Google Scholar 

  • Lafleur DL, Pittenger C, Kelmendi B et al (2006) N-Acetylcysteine augmentation in serotonin reuptake inhibitor refractory obsessive– compulsive disorder. Psychopharmacology 184:254–256

    Article  CAS  PubMed  Google Scholar 

  • Land JM, Mowbray J, Clark JB (1976) Control of pyruvate and h-hydroxybutyrate utilization in rat brain mitochondria and its relevance to phenylketonuria and maple syrup urine disease. J Neurochem 26:823–830

    Article  CAS  PubMed  Google Scholar 

  • Leslie SW, Brown LM, Trent RD et al (1992) Stimulation of N-methyl-D-aspartate receptor-mediated calcium entry into dissociated neurons by reduced and oxidized glutathione. Mol Pharmacol 41:308–314

    CAS  PubMed  Google Scholar 

  • Linck VM, Costa-Campos L, Pilz LK, Garcia CRL, Elisabetsky E (2012) AMPA glutamate receptors mediate the antidepressant-like effects of N-acetylcysteine in the mouse tail suspension test. Behav Pharmacol 23:171–177

    Article  CAS  PubMed  Google Scholar 

  • Lucca G, Comim CM, Valvassori SS et al (2008) Chronic mild stress paradigm reduces sweet food intake in rats without affecting brain derived neurotrophic factor protein levels. Curr Neurovasc Res 5:207–213

    Article  CAS  PubMed  Google Scholar 

  • Maes M, De Vos N, Pioli R et al (2000) Lower serum vitamin E concentrations in major depression. Another marker of lowered antioxidant defences in that illness J Affect Disord 58(3):241–246

    CAS  Google Scholar 

  • Maes M, Galecki P, Chang YS, Berk M (2011) A review on the oxidative and nitrosative stress (O&NS) pathways in major depression and their possible contribution to the (neuro) degenerative processes in that illness. Prog Neuropsychopharmacol Biol Psychiatry 35:676–92

    Article  CAS  PubMed  Google Scholar 

  • Maes M, Mihaylova I, Kubera M, Uytterhoeven M, Vrydags N, Bosmans E (2009a) Increased 8-hydroxy-deoxyguanosine, a marker of oxidative damage to DNA, in major depression and myalgic encephalomyelitis/chronic fatigue syndrome. Neuro Endocrinol Lett 30:715–722

    CAS  PubMed  Google Scholar 

  • Maes M, Mihaylova I, Kubera M, Uytterhoeven M, Vrydags N, Bosmans E (2009b) Lower plasma Coenzyme Q10 in depression: a marker for treatment resistance and chronic fatigue in depression and a risk factor to cardiovascular disorder in that illness. Neuro Endocinol Lett 30:462–469

    CAS  Google Scholar 

  • Magalhães PV, Dean OM, Bush AI et al (2011) N-acetyl cysteine add-on treatment for bipolar II disorder: a subgroup analysis of a randomized placebo-controlled trial. J Affect Disord 129:317–20

    Article  PubMed  Google Scholar 

  • Mescka C, Moraes T, Rosa A et al (2011) In vivo neuroprotective effect of L-carnitine against oxidative stress in maple syrup urine disease. Metab Brain Dis 26:21–28

    Article  CAS  PubMed  Google Scholar 

  • Mokoena ML, Harvey BH, Oliver DW, Brink CB (2010) Ozone modulates the effects of imipramine on immobility in the forced swim test, and nonspecific parameters of hippocampal oxidative stress in the rat. Metab Brain Dis 25:125–133

    Article  CAS  PubMed  Google Scholar 

  • Muelly ER, Moore GJ, Bunce SC et al (2013) Biochemical correlates of neuropsychiatric illness in maple syrup urine disease. J Clin Invest 123(4):1809–20

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Nestler EJ, Gould E, Manji H et al (2002) Preclinical model: status of basic research in depression. Biol Psychiatry 52:503–528

    Article  PubMed  Google Scholar 

  • Pilla C, Cardozo RF, Dutra-Filho CS, Wyse AT, Wajner M, Wannmacher CM (2003) Creatine kinase activity from rat brain is inhibited by branched-chain amino acids in vitro. Neurochem Res 28:675–679

    Article  CAS  PubMed  Google Scholar 

  • Porsolt RD, Le Pichon M, Jalfre M (1977) Depression: a new animal model sensitive to antidepressant treatments. Nature 21:266–730

    Google Scholar 

  • Prensky AL, Moser HW (1966) Brain lipids, proteolipids, and free amino acids in maple syrup urine disease. J Neurochem 13:863–874

    Article  CAS  PubMed  Google Scholar 

  • Prensky AL, Moser HW (1967) Changes in the amino acid composition of proteolipids of white matter during maturation of the human nervous system. J Neurochem 14:117–121

    Article  CAS  PubMed  Google Scholar 

  • Réus GZ, Stringari RB, de Souza B et al (2010) Harmine and imipramine promote antioxidant activities in prefrontal cortex and hippocampus. Oxid Med Cell Longev 3:325–331

    Article  PubMed Central  PubMed  Google Scholar 

  • Réus GZ, Stringari RB, Ribeiro KF et al (2011) Ketamine plus imipramine treatment induces antidepressant-like behavior and increases CREB and BDNF protein levels and PKA and PKC phosphorylation in rat brain. Behav Brain Res 221:166–171

    Article  PubMed  Google Scholar 

  • Ribeiro CA, Sgaravatti AM, Rosa RB et al (2008) Inhibition of brain energy metabolism by the branched-chain amino acids accumulating in maple syrup urine disease. Neurochem Res 33:114–124

    Article  CAS  PubMed  Google Scholar 

  • Scaini G, de Rochi N, Jeremias IC et al (2012) Evaluation of acetylcholinesterase in an animal model of maple syrup urine disease. Mol Neurobiol 45:279–86

    Article  CAS  PubMed  Google Scholar 

  • Scaini G, Comim CM, Oliveira GM et al (2013a) Chronic administration of branched-chain amino acids impairs spatial memory and increases brain-derived neurotrophic factor in a rat model. J Inherit Metab Dis 36:721–730

    Article  CAS  PubMed  Google Scholar 

  • Scaini G, Mello-Santos LM, Furlanetto CB et al (2013b) Acute and chronic administration of the branched-chain amino acids decreases nerve growth factor in rat hippocampus. Mol Neurobiol. doi:10.1007/s12035-013-8447-1

    Google Scholar 

  • Schönberger S, Schweiger B, Schwahn B, Schwarz M, Wendel U (2004) Dysmyelination in the brain of adolescents and young adults with maple syrup urine disease. Mol Genet Metab 82:69–75

    Article  PubMed  Google Scholar 

  • Sgaravatti AM, Rosa RB, Schuck PF et al (2003) Inhibition of brain energy metabolism by the a-keto acids accumulating in maple syrup urine disease. Biochim Biophys Acta 1639:232–238

    Article  CAS  PubMed  Google Scholar 

  • Smaga I, Pomierny B, Krzyżanowska W et al (2012) N-acetylcysteine possesses antidepressant-like activity through reduction of oxidative stress: behavioral and biochemical analyses in rats. Prog Neuropsychopharmacol Biol Psychiatry 39:280–87

    Article  CAS  PubMed  Google Scholar 

  • Strauss KA, Puffenberger EG, Morton DH (2006) Maple syrup urine disease. In: Pagon R, Bird T, Dolan C, Stephens K, Adam M (eds) GeneReviews. University of Washington, Seattle, Washington, USA

    Google Scholar 

  • Taketomi T, Kunishita T, Hara A, Mizushima S (1983) Abnormal protein and lipid compositions of the cerebral myelin of a patient with maple syrup urine disease. Jpn J Exp Med 53:109–116

    CAS  PubMed  Google Scholar 

  • Tavares RG, Santos CE, Tasca CI, Wajner M, Souza DO, Dutra-Filho CS (2000) Inhibition of glutamate uptake into synaptic vesicles of rat brain by the metabolites accumulating in maple syrup urine disease. J Neurol Sci 181:44–49

    Article  CAS  PubMed  Google Scholar 

  • Treacy E, Clow CL, Reade TR, Chitayat D, Mamer OA, Scriver CR (1992) Maple syrup urine disease: interrelationship between branched-chain amino-, oxo- and hydroxyacids; implications for treatment; associations with CNS dysmyelination. J Inherit Metab Dis 15:121–135

    Article  CAS  PubMed  Google Scholar 

  • Tribble D, Shapira R (1983) Myelin proteins: degradation in rat brain initiated by metabolites causative of maple syrup urine disease. Biochem Biophys Res Commun 114:440–446

    Article  CAS  PubMed  Google Scholar 

  • Tuon L, Comim CM, Antunes MM et al (2007) Imipramine reverses the depressive symptoms in sepsis survivor rats. Intensive Care Med 33:2165–2167

    Article  CAS  PubMed  Google Scholar 

  • Varga V, Jenei Z, Janáky R, Saransaari P, Oja SS (1997) Glutathione is an endogenous ligand of rat brain N-methyl-D-aspartate (NMDA) and 2-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors. Neurochem Res 22:1165–1171

    Article  CAS  PubMed  Google Scholar 

  • Venè R, Castellani P, Delfino L, Lucibello M, Ciriolo MR, Rubartelli A (2011) The cystine/cysteine cycle and GSH are independent and crucial antioxidant systems in malignant melanoma cells and represent druggable targets. Antioxid Redox Signal 15:2439–2453

    Article  PubMed  Google Scholar 

  • Wajner M, Coelho DM, Barschak AG et al (2000) Reduction of large neutral amino acid concentration in plasma and CSF of patients with maple syrup urine disease during crises. J Inherit Metab Dis 23:505–512

    Article  CAS  PubMed  Google Scholar 

  • Wajner M, Vargas CR (1999) Reduction of plasma concentrations of large neutral amino acids in patients with maple urine disease during crises. Arch Dis Child 80:579

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Walterfang M, Bonnot O, Mocellin R, Velakoulis D (2013) The neuropsychiatry of inborn errors of metabolism. J Inherit Metab Dis 36(4):687–702

    Article  CAS  PubMed  Google Scholar 

  • Willner P, Benton D, Brown E et al (1998) “Depression” increases “craving” for sweet rewards in animal and human models of depression and craving. Psychopharmacology 136:272–283

    Article  CAS  PubMed  Google Scholar 

  • Willner P, Towell A, Sampson D, Sophokleous S, Muscat R (1987) Reduction of sucrose preference by chronic unpredictable mild stress, and its restoration by a tricyclic antidepressant. Psychopharmacology 93:358–364

    Article  CAS  PubMed  Google Scholar 

  • Yagasaki Y, Numakawa T, Kumamaru E, Hayashi T, Su TP, Kunugi H (2006) Chronic antidepressants potentiate via sigma-1 receptors the brain-derived neurotrophic factor-induced signaling for glutamate release. J Biol Chem 281:12941–12949

    Article  CAS  PubMed  Google Scholar 

  • Yudkoff M, Daikhin Y, Lin ZP et al (1994) Interrelationships of leucine and glutamate metabolism in cultured astrocyts. J Neurochem 62:1192–1202

    Article  CAS  PubMed  Google Scholar 

  • Zielke HR, Zielke CL, Baab PJ, Collins RM (2002) Large neutral amino acids auto exchange when infused by microdialysis into the rat brain: implications for maple syrup urine disease and phenylketonuria. Neurochem Int 40:347–54

    Article  PubMed  Google Scholar 

  • Zinnanti WJ, Lazovic J, Griffin K et al (2009) Dual mechanism of brain injury and novel treatment strategy in maple syrup urine disease. Brain 132:903–918

    Article  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by grants from Programa de Pós-graduação em Ciências da Saúde – Universidade do Extremo Sul Catarinense (UNESC) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Laboratório de Bioenergética, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense Criciúma, Av. UniversitÄria, 1105, Criciúma, 88806-000, SC, Brazil

    Giselli Scaini, Gabriela C. Jeremias, Camila B. Furlanetto & Emilio L. Streck

  2. Instituto Nacional de Ciência e Tecnologia Translacional em Medicina (INCT-TM), Porto Alegre, RS, Brazil

    Giselli Scaini, Gabriela C. Jeremias, Camila B. Furlanetto, Diogo Dominguini, Clarissa M. Comim, João Quevedo & Emilio L. Streck

  3. Laboratório de Neurociências, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil

    Diogo Dominguini, Clarissa M. Comim & João Quevedo

  4. Laboratório de Erros Inatos do Metabolismo, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil

    Patrícia F. Schuck & Gustavo C. Ferreira

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  1. Giselli Scaini
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  2. Gabriela C. Jeremias
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  3. Camila B. Furlanetto
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  9. Emilio L. Streck
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Correspondence to Emilio L. Streck .

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Editors and Affiliations

  1. Division of Human Genetics, Medical University Innsbruck, Innsbruck, Austria

    Johannes Zschocke

  2. Clinical Pharmacology Unit, WSU Division of Health Sciences, Spokane, USA

    K. Michael Gibson

  3. Department of Biochemistry Genetics Unit, University of Oxford, Oxford, United Kingdom

    Garry Brown

  4. Department of Pediatrics IGMD, Tulane University Medical Center, New Orleans, Louisiana, USA

    Eva Morava

  5. Center for Child and Adolescent Medicine Heidelberg University Hospital, Heidelberg, Germany

    Verena Peters

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Communicated by: Bruce A Barshop, MD, PhD

Appendices

Author Contribution

Giselli Scaini, Emilio L. Streck, and João Quevedo designed the study and wrote the protocol. Giselli Scaini, Gabriela C. Jeremias, Camila B. Furlanetto, and Diogo Dominguini conducted the experiments. Giselli Scaini and Clarissa M. Comim undertook the statistical analysis; and Giselli Scaini and Emilio L. Streck wrote the first draft of the manuscript. All authors contributed to and have approved the final manuscript.

Conflicts of Interest

Giselli Scaini, Gabriela C. Jeremias, Camila B. Furlanetto, Diogo Dominguini, Clarissa M. Comim, João Quevedo, Patrícia F. Schuck, Gustavo C. Ferreira, and Emilio L. Streck declare that they have no conflict of interest.

Informed Consent and Animal Rights

All institutional and national guidelines for the care and use of laboratory animals were followed. All experimental procedures were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and the Brazilian Society for Neuroscience and Behavior recommendations for animal care.

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Scaini, G. et al. (2013). Behavioral Responses in Rats Submitted to Chronic Administration of Branched-Chain Amino Acids. In: Zschocke, J., Gibson, K., Brown, G., Morava, E., Peters, V. (eds) JIMD Reports - Case and Research Reports, Volume 13. JIMD Reports, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8904_2013_274

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