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Electron Microscopy Protocols for the Study of Hydrocarbon-Producing and Hydrocarbon-Decomposing Microbes: Classical and Advanced Methods

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Hydrocarbon and Lipid Microbiology Protocols

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

One of the fascinating areas of hydrocarbon microbiology biology is the quest for an ultratstructural understanding of (macro)-molecular mechanisms underlying the degradation, synthesis, and intracellular storage of hydrocarbons, which due to their hydrophobic characteristics continuously threaten the integrity of biological membranes. Here we review classical and novel advanced electron microscopy approaches, including correlative light and electron microscopy that in combination with genetics and biochemical experimentation can be utilized to study such hydrocarbon–cell interactions.

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Author information

Authors and Affiliations

  1. Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, MS Donner, Berkeley, CA, 94720, USA

    Kamna Jhamb & Manfred Auer

Authors
  1. Kamna Jhamb
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  2. Manfred Auer
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Corresponding author

Correspondence to Manfred Auer .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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Jhamb, K., Auer, M. (2015). Electron Microscopy Protocols for the Study of Hydrocarbon-Producing and Hydrocarbon-Decomposing Microbes: Classical and Advanced Methods. In: McGenity, T., Timmis, K., Nogales, B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2015_96

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  • DOI: https://doi.org/10.1007/8623_2015_96

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-49132-4

  • Online ISBN: 978-3-662-49134-8

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