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Primary Human Leukemia Stem Cell (LSC) Isolation and Characterization

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Stem Cells and Lineage Commitment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2736))

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Abstract

Leukemia stem cells (LSC) are thought to be the basis of leukemia progression since they are highly resistant to conventional chemotherapy. LSC isolation is critical in experimental studies, drug development, and application. Due to their likely hematopoietic stem cell (HSC) origin, LSCs have surface antigens that are similar to HSC. Surface markers such as CD34, CD123, CD133, and CD33 have been used extensively to assess LSCs. LSCs could be separated from other cells using magnetic selection (MS) or flow cytometry selection (FCS) methods using these markers. Understanding the role of LSCs in cancer progression and how to therapeutically target them in vitro and in vivo is critical for the development of LSC-targeting drug candidates. In this chapter, we set out to describe the primary human LSC purification and characterization processes used on patient samples with leukemia and lymphoma.

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Acknowledgments

This study was supported by funds provided by Gilead Sciences International Hematology and Oncology program. NM and FK were supported by “Gilead ile Hayat Bulan Fikirler.”

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Authors and Affiliations

  1. Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Türkiye

    Neslihan Meriç & Fatih Kocabaş

  2. Faculty of Engineering and Life Sciences, Kütahya Health Sciences University, Kütahya, Türkiye

    Neslihan Meriç

Authors
  1. Neslihan Meriç
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  2. Fatih Kocabaş
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Corresponding authors

Correspondence to Neslihan Meriç or Fatih Kocabaş .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Meriç, N., Kocabaş, F. (2023). Primary Human Leukemia Stem Cell (LSC) Isolation and Characterization. In: Turksen, K. (eds) Stem Cells and Lineage Commitment. Methods in Molecular Biology, vol 2736. Humana, New York, NY. https://doi.org/10.1007/7651_2023_497

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  • DOI: https://doi.org/10.1007/7651_2023_497

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3536-0

  • Online ISBN: 978-1-0716-3537-7

  • eBook Packages: Springer Protocols

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