Abstract
Here, we describe a protocol for reprogramming of bone marrow–derived multipotent mesenchymal stromal/stem cells to obtain induced pluripotent stem cells from patients with primary immune deficiencies using lentiviral vectors, followed by hematopoietic differentiation of the MSC-derived iPSCs. This protocol is particularly helpful in cases where it is difficult to obtain sufficient numbers of hematopoietic cells for research and can be applied to model any hematological/immunological disease.
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Acknowledgments
This work was supported by a grant from The Scientific and Technological Research Council of Turkey (TÜBİTAK), project no. 214S071.
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Güney-Esken, G., Aerts-Kaya, F. (2021). Generation and Hematopoietic Differentiation of Mesenchymal Stromal/Stem Cell-Derived Induced Pluripotent Stem Cell Lines for Disease Modeling of Hematopoietic and Immunological Diseases. In: Turksen, K. (eds) Induced Pluripotent Stem Cells and Human Disease. Methods in Molecular Biology, vol 2549. Humana, New York, NY. https://doi.org/10.1007/7651_2021_452
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DOI: https://doi.org/10.1007/7651_2021_452
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2584-2
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