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Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells

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Book cover Embryonic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2520))

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Abstract

Mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) recapitulate in vitro the epiblast first cell lineage decision, providing a powerful tool to investigate the mechanisms underlying the pluripotent state transition. Here, we describe a defined and robust protocol to transiently induce mEpiLCs from mESCs, together with a concise overview for their unbiased characterization for subsequent downstream applications.

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References

  1. Evans MJ, Kaufman MH (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292:154–156

    Article  CAS  PubMed  Google Scholar 

  2. Hayashi K, Ohta H, Kurimoto K et al (2011) Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells. Cell 146:519–532

    Article  CAS  PubMed  Google Scholar 

  3. Nichols J, Smith A (2009) Naive and primed pluripotent states. Cell Stem Cell 4:487–492

    Article  CAS  PubMed  Google Scholar 

  4. Smith A (2017) Formative pluripotency: The executive phase in a developmental continuum. Development 144:365–373

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Ying Q-L, Smith A (2017) The art of capturing pluripotency: creating the right culture. Stem cell Rep 8:1457–1464

    Article  Google Scholar 

  6. Du P, Pirouz M, Choi J et al (2018) An intermediate pluripotent state controlled by microRNAs is required for the naive-to-primed stem cell transition. Cell Stem Cell 22:851–864

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Pecori F, Yokota I, Hanamatsu H et al (2021) A defined glycosylation regulatory network modulates total glycome dynamics during pluripotency state transition. Sci Rep 11:1276

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Sperber H, Mathieu J, Wang Y et al (2015) The metabolome regulates the epigenetic landscape during naive-to-primed human embryonic stem cell transition. Nat Cell Biol 17:1523–1535

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Niwa H, Ogawa K, Shimosato D et al (2009) A parallel circuit of LIF signalling pathways maintains pluripotency of mouse ES cells. Nature 460:118–122

    Article  CAS  PubMed  Google Scholar 

  10. Lanner F, Rossant J (2010) The role of FGF/Erk signaling in pluripotent cells. Development 137:3351–3360

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number JP18K06139 (to S.N.) and Japan Science and Technology Agency (JST)-Mirai Program Grant Number JPMJMI18GB (to S.N.). F.P. was supported by Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) Scholarship (Top Global University Project).

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Authors and Affiliations

  1. Laboratory of Cell Biology, Department of Bioinformatics, Graduate School of Engineering, Soka University, Tokyo, Japan

    Federico Pecori & Shoko Nishihara

  2. Glycan & Life System Integration Center (GaLSIC), Soka University, Tokyo, Japan

    Shoko Nishihara

Authors
  1. Federico Pecori
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  2. Shoko Nishihara
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Corresponding author

Correspondence to Shoko Nishihara .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Pecori, F., Nishihara, S. (2021). Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells. In: Turksen, K. (eds) Embryonic Stem Cell Protocols . Methods in Molecular Biology, vol 2520. Humana, New York, NY. https://doi.org/10.1007/7651_2021_403

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  • DOI: https://doi.org/10.1007/7651_2021_403

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2436-4

  • Online ISBN: 978-1-0716-2437-1

  • eBook Packages: Springer Protocols

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