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Feeder-Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol

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Embryonic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2520))

Abstract

Mouse embryonic stem cells (mESCs) were first derived and cultured nearly 30 years ago and have been beneficial tools to create transgenic mice and to study early mammalian development so far. Fibroblast feeder cell layers are often used at some stage in the culture protocol of mESCs. The feeder layer—often mouse embryonic fibroblasts (MEFs)—contribute to the mESC culture as a substrate to increase culture efficiency, maintain pluripotency, and facilitate survival and growth of the stem cells. Various feeder-dependent and feeder-independent culture and differentiation protocols have been established for mESCs. Here we describe the isolation, culture, and preparation feeder cell layers and establishment of feeder-dependent/independent protocol for mESC culture. In addition, basic mESC protocols for culture, storage, and differentiation were described.

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Acknowledgments

This study was supported by TÜBİTAK 2232 International Fellowship for Outstanding Researchers Program (Project no: 118C186). The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey

    Hatice Burcu Şişli, Selinay Şenkal, Derya Sağraç, Taha Bartu Hayal & Ayşegül Doğan

Authors
  1. Hatice Burcu Şişli
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  2. Selinay Şenkal
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  3. Derya Sağraç
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  4. Taha Bartu Hayal
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  5. Ayşegül Doğan
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Corresponding author

Correspondence to Ayşegül Doğan .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Şişli, H.B., Şenkal, S., Sağraç, D., Hayal, T.B., Doğan, A. (2021). Feeder-Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol. In: Turksen, K. (eds) Embryonic Stem Cell Protocols . Methods in Molecular Biology, vol 2520. Humana, New York, NY. https://doi.org/10.1007/7651_2021_402

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  • DOI: https://doi.org/10.1007/7651_2021_402

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2436-4

  • Online ISBN: 978-1-0716-2437-1

  • eBook Packages: Springer Protocols

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