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Rapid Evaluation of Intestinal Paracellular Permeability Using the Human Enterocytic-Like Caco-2/TC7 Cell Line

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Permeability Barrier

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2367))

Abstract

Paracellular permeability of the intestinal epithelium is a feature of the intestinal barrier, which plays an important role in the physiology of gut and the whole organism. Intestinal paracellular permeability is controlled by complex processes and is involved in the passage of ions and fluids (called pore pathway) and macromolecules (called leak pathway) through tight junctions, which seal the intercellular space. Impairment of intestinal paracellular permeability is associated with several diseases. The identification of a defect in intestinal paracellular permeability may help to understand the implication of gut barrier as a cause or a consequence in human pathology. Here we describe two complementary methods to evaluate alteration of paracellular permeability in cell culture, using the human intestinal cell line Caco-2 and its clone Caco-2/TC7.

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Acknowledgments

This work was supported by: the Brazilian government’s Science Without Borders Program, the Association François Aupetit (AFA), Institut National de la Santé et de la Recherche Médicale, Sorbonne Université, Ecole Pratique des Hautes Etudes. B.G.P. received a doctoral fellowship (CNPq 207303/2014-2). D.A. received a fellowship from CORDDIM Ile de France.

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Authors and Affiliations

  1. Centre de Recherche sur l’Inflammation, INSERM UMR1149, Université de Paris, Paris, France

    Bárbara Graziela Postal

  2. Biology and Genetics of Bacterial Cell Wall Unit, Pasteur Institute, Paris, France

    Bárbara Graziela Postal

  3. Centre de Recherche Saint-Antoine, INSERM UMRS 938, Sorbonne Université, INSERM, Paris, France

    Doriane Aguanno, Sophie Thenet & Véronique Carrière

  4. EPHE, PSL University, Paris, France

    Sophie Thenet

  5. Center for Microbiome Medicine (PaCeMM) FHU, AP-HP, Paris, Ile-de-France, France

    Sophie Thenet & Véronique Carrière

Authors
  1. Bárbara Graziela Postal
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  2. Doriane Aguanno
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  3. Sophie Thenet
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  4. Véronique Carrière
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Corresponding author

Correspondence to Véronique Carrière .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Postal, B.G., Aguanno, D., Thenet, S., Carrière, V. (2021). Rapid Evaluation of Intestinal Paracellular Permeability Using the Human Enterocytic-Like Caco-2/TC7 Cell Line. In: Turksen, K. (eds) Permeability Barrier. Methods in Molecular Biology, vol 2367. Humana, New York, NY. https://doi.org/10.1007/7651_2021_366

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  • DOI: https://doi.org/10.1007/7651_2021_366

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1672-7

  • Online ISBN: 978-1-0716-1673-4

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