Abstract
The small intestine plays roles in the absorption and metabolism of orally administered drugs and chemicals. Tight junctions between intestinal epithelial cells, which form a tight barrier preventing the invasion of pathogens and toxins, are essential components of the intestinal defense system. These intestinal functions have generally been evaluated using established cell lines or primary cells in two-dimensional culture. However, these culture systems have not shown the complexity of the three-dimensional structure and diversity of cell types comprising the intestinal epithelial tissue. Here, we report the generation of intestinal organoids using human induced pluripotent stem cells subjected to sequential treatment with different cytokines and compounds. We further describe the tool for evaluating intestinal barrier functions using organoids as a physiologically relevant human platform.
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Acknowledgements
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (#17H04107 and #17K19503 to Y. K., #17K00576 to S. Y.), the Research on Regulatory Harmonization and Evaluation of Pharmaceuticals, Medical Devices, Regenerative and Cellular Therapy Products, Gene Therapy Products, and Cosmetics from Japan Agency for Medical Research and Development, AMED (JP18mk0104117 to Y. K.), and a grant from the Smoking Research Foundation (Y. K.).
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Yamada, S., Kanda, Y. (2021). Evaluation of Barrier Functions in Human iPSC-Derived Intestinal Epithelium. In: Turksen, K. (eds) Permeability Barrier. Methods in Molecular Biology, vol 2367. Humana, New York, NY. https://doi.org/10.1007/7651_2021_346
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DOI: https://doi.org/10.1007/7651_2021_346
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1672-7
Online ISBN: 978-1-0716-1673-4
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