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Stem Cells and Aging pp 155–165Cite as

3D-Embedded Cell Cultures to Study Tendon Biology

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2045))

Abstract

Tendons harbor various cell populations, including cells displaying classical adult mesenchymal stromal cell criteria. Previous studies have shown that a tenogenic phenotype is more effectively maintained in a 3D cell culture model under mechanical load. This chapter describes a method to isolate tendon-derived cells from rat Achilles tendons and the subsequent formation of 3D-embedded cell cultures. These tendon-like constructs can then be analyzed by various means, including histology, immunohistochemistry, qPCR, or standard protein analysis techniques.

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Authors and Affiliations

  1. Institute of Tendon and Bone Regeneration, Paracelsus Medical University – Spinal Cord Injury and Tissue Regeneration Center Salzburg, Salzburg, Austria

    Renate Gehwolf, Gabriel Spitzer, Andrea Wagner, Christine Lehner, Nadja Weissenbacher, Herbert Tempfer & Andreas Traweger

  2. Austrian Cluster for Tissue Regeneration, Vienna, Austria

    Renate Gehwolf, Gabriel Spitzer, Andrea Wagner, Christine Lehner, Nadja Weissenbacher, Herbert Tempfer & Andreas Traweger

Authors
  1. Renate Gehwolf
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  2. Gabriel Spitzer
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  3. Andrea Wagner
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  4. Christine Lehner
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  5. Nadja Weissenbacher
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  6. Herbert Tempfer
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  7. Andreas Traweger
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Corresponding author

Correspondence to Renate Gehwolf .

Editor information

Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Gehwolf, R. et al. (2019). 3D-Embedded Cell Cultures to Study Tendon Biology. In: Turksen, K. (eds) Stem Cells and Aging . Methods in Molecular Biology, vol 2045. Humana, New York, NY. https://doi.org/10.1007/7651_2019_208

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  • DOI: https://doi.org/10.1007/7651_2019_208

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9712-1

  • Online ISBN: 978-1-4939-9713-8

  • eBook Packages: Springer Protocols

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