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A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration

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Decellularized Scaffolds and Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1577))

Abstract

Traditional ex vivo expansion of adult stem cells yields an insufficient quantity of less potent cells. Here we describe the fabrication of decellularized matrix deposited by synovium-derived stem cells (SDSCs). This matrix could serve as a three-dimensional expansion system to rejuvenate cells for proliferation and tissue-specific differentiation potential, which could benefit cartilage regeneration. The decellularized stem cell matrix (DSCM) might be a powerful system for tissue engineering and regeneration.

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Acknowledgements

We thank Suzanne Danley for editing the manuscript. This project was partially supported by Research Grants from the Musculoskeletal Transplant Foundation (MTF) and the National Institutes of Health (AR062763-01A1 and AR067747-01A1).

Author information

Authors and Affiliations

  1. Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, Division of Exercise Physiology, and Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV, USA

    Jingting Li

  2. Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, PO Box 9196, One Medical Center Drive, Morgantown, WV, 26506-9196, USA

    Ming Pei

Authors
  1. Jingting Li
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  2. Ming Pei
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Corresponding author

Correspondence to Ming Pei .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2017 Springer Science+Business Media New York

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Li, J., Pei, M. (2017). A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration. In: Turksen, K. (eds) Decellularized Scaffolds and Organogenesis. Methods in Molecular Biology, vol 1577. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_27

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  • DOI: https://doi.org/10.1007/7651_2017_27

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7655-3

  • Online ISBN: 978-1-4939-7656-0

  • eBook Packages: Springer Protocols

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