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Organoids pp 123–133Cite as

Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-recombinase-Mediated Gene Deletion

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1576))

Abstract

The discovery of Lgr5 as a marker of adult stem cells meant that stem cell populations could be purified and studied in isolation. Importantly, when cultured under the appropriate conditions these stem cells form organoids in tissue culture that retain many features of the tissue of origin. The organoid cultures are accessible to genetic and biochemical manipulation, bridging the gap between in vivo mouse models and conventional tissue culture. Here we describe robust protocols to establish organoids from gastrointestinal tissues (stomach, intestine, liver) and Cre-recombinase mediated gene manipulation in vitro.

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Authors and Affiliations

  1. Molecular Oncology Laboratory, University of Melbourne, Melbourne, VIC, 3000, Australia

    Dustin J. Flanagan, Renate H. M. Schwab, Bang M. Tran, Toby J. Phesse & Elizabeth Vincan

  2. Victorian Infectious Diseases Reference Laboratory, Doherty Institute, 792 Elizabeth Street, Melbourne, VIC, 3000, Australia

    Dustin J. Flanagan, Renate H. M. Schwab, Bang M. Tran, Toby J. Phesse & Elizabeth Vincan

  3. School of Biomedical Sciences, Curtin University, Perth, WA, 6845, Australia

    Elizabeth Vincan

Authors
  1. Dustin J. Flanagan
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  2. Renate H. M. Schwab
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  3. Bang M. Tran
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  4. Toby J. Phesse
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  5. Elizabeth Vincan
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Corresponding author

Correspondence to Elizabeth Vincan .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, ON, Canada

    Kursad Turksen

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© 2016 Springer Science+Business Media New York

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Flanagan, D.J., Schwab, R.H.M., Tran, B.M., Phesse, T.J., Vincan, E. (2016). Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-recombinase-Mediated Gene Deletion. In: Turksen, K. (eds) Organoids. Methods in Molecular Biology, vol 1576. Humana, New York, NY. https://doi.org/10.1007/7651_2016_14

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  • DOI: https://doi.org/10.1007/7651_2016_14

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7616-4

  • Online ISBN: 978-1-4939-7617-1

  • eBook Packages: Springer Protocols

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