Skip to main content
SpringerLink
Log in

Capture and Isolation of Circulating Melanoma Cells Using Photoacoustic Flowmetry

  • Protocol
  • First Online:
Methods in Molecular Biology

Part of the book series: Methods in Molecular Biology

Abstract

Circulating tumor cells (CTCs) are those cells that separate from a solid tumor and spread through the blood or lymphatic systems. While there are many open questions concerning the biology of CTCs, there is mounting evidence that some of these cells go on to create secondary tumors in distant organs, thus enabling metastatic disease. Detection of CTCs may have clinical impact by providing prognostic information. Furthermore, molecular and genetic analysis of CTCs may enable cancer biologists to answer questions about the metastatic process, such as whether these cells undergo epithelial–mesenchymal transition. Using a photoacoustic flowmeter, in which we induce ultrasonic responses from circulating melanoma cells (CMCs), we identify, capture, and isolate these cells for further analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

References

  1. Paterlini-Brechot P, Benali N (2007) Circulating tumor cells (CTC) detection: clinical impact and future directions. Cancer Lett 253(2):180–204

    Article  CAS  PubMed  Google Scholar 

  2. Cristofanilli M, Braun S (2010) Circulating tumor cells revisited. JAMA 303(11):1092

    Article  PubMed  Google Scholar 

  3. Gage T, Fan S (2010) What goes around, comes around: a review of circulating tumor cells. Analysis 11:18

    Google Scholar 

  4. Chang Y, Ye J, Thomas T, Cao Z, Kotlyar A, Tkaczyk E, Baker J Jr, Norris T (2010) Fiber-optic multiphoton flow cytometry in whole blood and in vivo. J Biomed Opt 15:047004

    Article  PubMed Central  PubMed  Google Scholar 

  5. Okegawa T, Hayashi K, Hara H, Nutahara K, Higashihara E (2010) Immunomagnetic quantification of circulating tumor cells in patients with urothelial cancer. Int J Urol 17(3):254–258

    Article  PubMed  Google Scholar 

  6. Xu T, Lu B, Tai Y, Goldkorn A (2010) A cancer detection platform which measures telomerase activity from live circulating tumor cells captured on a microfilter. Cancer Res 70(16):6420

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Riethdorf S, Fritsche H, Muller V, Rau T, Schindlbeck C, Rack B, Janni W, Coith C, Beck K, Janicke F, Jackson S, Gornet T, Cristofanilli M, Pantel K (2007) Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the cell search system. Clin Cancer Res 13(3):920–928

    Article  CAS  PubMed  Google Scholar 

  8. Zheng S, Lin H, Liu J, Balic M, Datar R, Cote R, Tai Y (2007) Membrane microfilter device for selective capture, electrolysis and genomic analysis of human circulating tumor cells. J Chromatogr 1162(2):154–161

    Article  CAS  Google Scholar 

  9. Zabaglo L, Ormerod M, Parton M, Ring A, Smith I, Dowsett M (2003) Cell filtration-laser scanning cytometry for the characterization of circulating breast cancer cells. Cytometry A 55A:102–108

    Article  Google Scholar 

  10. Gogas H, Kefala G, Bafaloukus D, Frangia K, Polyzos A, Pectasides D, Tsoutsos D, Panagiotou P, Ioannovich J, Loukopoulus D (2010) Prognostic significance of the sequential detection of circulating melanoma cells by rt-pcr in high-risk melanoma patients receiving adjuvant interferon. Br J Cancer 88(6):981–982

    Google Scholar 

  11. Pellegrino D, Bellina C, Manca G, Boni G, Grosso M, Volterrani D, Desideri I, Bianchi F, Bottoni A, Ciliberti V, Salimbeni G, Gandini D, Castagna M, Zucchi V, Romanini A (2000) Detection of melanoma cells in peripheral blood and sentinel lymph nodes by rt-pcr analysis: a comparative study with immunochemistry. Tumori 86(4):336–338

    CAS  PubMed  Google Scholar 

  12. Berking C, Schlupen E, Schraeder A, Atzpodien J, Volkenandt M (1999) Tumor markers in peripheral blood of patients with malignant melanoma: multimarker RT-PCR versus a luminoimmunometric assay for S-100. Arch Dermatol Res 291(9):479–484

    Article  CAS  PubMed  Google Scholar 

  13. Weight R, Dale P, Caldwell C, Lisle A, Viator J (2006) Photoacoustic detection of metastatic melanoma cells in the human circulatory system. Opt Lett 31:2998–3000

    Article  ADS  PubMed  Google Scholar 

  14. Gutiérrez-Juárez G, Gupta S, Weight R, Polo-Parada L, Papagiorgio C, Bunch J, Viator J (2010) Optical photoacoustic detection of circulating melanoma cells in vitro. Int J Thermophys 31(4):784–792

    Article  PubMed Central  ADS  PubMed  Google Scholar 

  15. Gutierrez-Juarez G, Gupta S, Al-Shaer M, Polo-Parada L, Dale P, Papageorgio C, Viator J (2010) Detection of melanoma cells in vitro using an optical detector of photoacoustic waves. Lasers Surg Med 42(3):274–281

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We acknowledge the support of the Department of Biological Engineering and the Christopher S. Bond Life Sciences Center at the University of Missouri. We acknowledge NIH R01 CA182840-01 for supporting this work.

Author information

Authors and Affiliations

  1. Biomedical Engineering, Duquesne University, Pittsburgh, PA, USA

    Benjamin S. Goldschmidt & John A. Viator

Authors
  1. Benjamin S. Goldschmidt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John A. Viator
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to John A. Viator .

Rights and permissions

Reprints and permissions

Copyright information

© 2015

About this protocol

Cite this protocol

Goldschmidt, B.S., Viator, J.A. (2015). Capture and Isolation of Circulating Melanoma Cells Using Photoacoustic Flowmetry. In: Methods in Molecular Biology. Humana Press. https://doi.org/10.1007/7651_2015_306

Download citation

  • DOI: https://doi.org/10.1007/7651_2015_306

  • Published:

  • Publisher Name: Humana Press

Publish with us

Policies and ethics

Search

Navigation