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Design and Development of In Vivo Sensor Systems: The Long and Tortured Road to a Self-Contained, Implantable Glucose Sensor for Diabetes Management

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Designing Receptors for the Next Generation of Biosensors

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Abbreviations

ARS:

Alizarin Red S; also known as 3,4-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt

DBA:

Dendrimer-boronic acid

IC50 :

Half Maximal Inhibitory Concentration

iDIOL:

Immobilized diol/saccharide analogue

Keq:

Equilibrium constant

MIP:

Moleculary imprinted polymer

NIR:

Near-infrared

PET:

Photoinduced electron transfer

pK a :

The negative logarithm of the dissociation constant

RFID:

Radio frequency identification

DBA:

A dendrimer construct that is functionalized with boronic acid receptor ligands and a fluorescent reporter moiety. The DBA is the sensing system signaling component that can competitively bind to the glucose analyte and to the 1,2- and 1,3-dihydroxy motif(s) of iDIOLs.

diol:

A saccharide analogue moiety that typically contains a 1,2- or 1,3-dihydroxy motif and a functional group that can be used for covalent immobilization of the diol on a support to create an iDIOL.

iDIOL:

An immobilized diol/saccharide analogue that contains a 1,2- or 1,3-dihydroxy moiety that is covalently attached to a support. The iDIOL competes with glucose for DBA binding, which produces a bound versus free sensing system signal.

DBA:glucose:iDIOL:

Designation of the three component competitive system where glucose and the iDIOL compete for DBA binding to produce a signal response that is proportional to glucose concentration.

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Acknowledgments

We thank PositiveID Corporation for their partial financial support for this project.

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  1. RECEPTORS LLC, Suite 510/MD 57, 1107 Hazeltine Boulevard, Chaska, MN, 55318-2533, USA

    Christina Thomas, Rachel Weller Roska & Robert E. Carlson

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  1. Christina Thomas
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Correspondence to Robert E. Carlson .

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  1. , Cranfield Health, Cranfield University, Cranfield Campus, Cranfield, MK 43 0AL, Bedfordshire, United Kingdom

    Sergey A. Piletsky

  2. , Cranfield Health, Cranfield University, Cranfield Campus, Cranfield, MK43 0AL, Bedfordshire, United Kingdom

    Michael J. Whitcombe

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Thomas, C., Roska, R.W., Carlson, R.E. (2012). Design and Development of In Vivo Sensor Systems: The Long and Tortured Road to a Self-Contained, Implantable Glucose Sensor for Diabetes Management. In: Piletsky, S., Whitcombe, M. (eds) Designing Receptors for the Next Generation of Biosensors. Springer Series on Chemical Sensors and Biosensors, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/5346_2012_23

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