Abstract
A comparative study on the basic structural characteristics of exopolysaccharide (EPS) and capsular polysaccharide (CPS) produced by the Southern Ocean (Indian Sector) origin Pseudoalteromonas sp. MB-16 has been conducted. It has a higher production rate of EPS as compared to that of CPS and the maximum production rate is 0.7 g/l when glucose is the carbon source. Elemental analysis and infrared spectroscopic study have given a brief compositional insight on the EPS and CPS produced by the marine bacterium, whereas scanning electron micrographs have revealed the morphological structure of Pseudoalteromonas sp. derived EPS and CPS. Both the polysaccharides show significant thermal stability as confirmed by thermogravimetric analysis. In all the experiments, Pseudoalteromonas sp. derived EPS and CPS have given similar results when compared to standard bacterial polysaccharides dextran and guar gum. This present study gave an important preliminary idea about the nature of bacterial polysaccharides produced by the Southern Ocean (Indian Sector) origin microbe.
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Acknowledgements
The authors are thankful for the Expedition support to MoES, New Delhi and NCAOR, Goa (No. MoES/NCAOR/SOS/1/2007PC-I; dated January 4, 2011) and research support to BIT Mesra, Ranchi. One of the authors gratefully acknowledges the financial support as M. Tech fellowship to Centre of Excellence (COE) (Ref No. NPIU/TEQIP II/FIN/31/158, dated April 16, 2013) at the Department of BioEngineering. Another author is thankful to SRF (State Funded) for the financial assistance [Fc (Sc.)/RS/SF/BOT./2014-15/103 (3)]. Urmi Halder is thankful for the financial assistance to JRF-State Fund [Fc (Sc.) /RS/SF/BOT./2017-18/22)].
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Significance Statement
First report on bacterial EPS/CPS production from Southern Ocean (Indian Sector). Optimization of EPS/CPS production. Preliminary characterization of both EPS and CPS from Pseudoalteromonas sp. MB-16.
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Sengupta, S., Banerjee, A., Halder, U. et al. Comparative Study on Structure of Exopolysaccharide and Capsular Polysaccharide Produced by Southern Ocean Origin Pseudoalteromonas sp. MB-16. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 283–290 (2019). https://doi.org/10.1007/s40011-017-0942-0
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DOI: https://doi.org/10.1007/s40011-017-0942-0