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Understanding the mechanisms for the lower nitrous oxide emissions from fodder beet urine compared with kale urine from dairy cows

  • Soils, Sec 1 • Soil Organic Matter Dynamics and Nutrient Cycling • Research Article
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Abstract

Purpose

Nitrous oxide (N2O) is a potent greenhouse gas and is produced in the soil by nitrification and denitrification processes. Previous studies have reported apparent lower N2O emissions from dairy cow urine from fodder beet plants than from kale plants, although the underpinning mechanisms were poorly understood. Here, we report a laboratory incubation study to improve understanding of possible mechanisms underpinning different N2O emissions from two different forage plants, fodder beet (FB) and kale.

Materials and methods

The treatments included two urine sources from cows grazing FB or kale and two soils (FB soil and kale soil): FB soil control, FB soil + FB urine, FB soil + kale urine, kale soil control, kale soil + FB urine and kale soil + kale urine. The incubation temperature was 10 °C to simulate New Zealand autumn/winter soil temperatures.

Results and discussion

Results showed that the total N2O emissions from FB urine treatment and from urine treatments in the FB soil were lower than those from kale urine in the kale soil (P < 0.05). There was a delay in the ammonia-oxidising bacteria (AOB) growth in the FB soil + urine compared with that in kale soil with urine and this led to slower ammonia oxidation in the FB soil. Denitrifier communities were not significantly affected by the urine or soil treatments.

Conclusions

These results suggest that there are plant secondary metabolites (PSMs) in the FB urine or root exudates in the FB soil, which might have inhibited the AOB growth and the nitrification process which led to lower N2O emissions in the FB soil or urine. Further research is needed to determine the chemical composition of urine from different forage plants and to identify potential PSMs or root exudates in the urine or soil from different forage plants.

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References

  • Baggs EM, Smales CL, Bateman EJ (2010) Changing pH shifts the microbial source as well as the magnitude of N2O emission from soil. Biol Fert Soil 46:793–805

    Article  CAS  Google Scholar 

  • Bourgaud F, Gravot A, Milesi S, Gontier E (2001) Production of plant secondary metabolites: a historical perspective. Plant Sci 161:839–851

    Article  CAS  Google Scholar 

  • Cameron K, Di H, Moir J (2013) Nitrogen losses from the soil/plant system: a review. Ann Appl Biol 162:145–173

    Article  CAS  Google Scholar 

  • De Klein C, Letica S, Macfie P (2014) Evaluating the effects of dicyandiamide (DCD) on nitrogen cycling and dry matter production in a 3-year trial on a dairy pasture in South Otago, New Zealand. New Zeal J Agr Res 57:316–331

    Article  Google Scholar 

  • Di HJ, Cameron KC (2002) The use of a nitrification inhibitor, dicyandiamide (DCD), to decrease nitrate leaching and nitrous oxide emissions in a simulated grazed and irrigated grassland. Soil Use Manag 18:395–403

    Article  Google Scholar 

  • Di HJ, Cameron KC (2004) Treating grazed pasture soil with a nitrification inhibitor, eco-n™, to decrease nitrate leaching in a deep sandy soil under spray irrigation—a lysimeter study. NZ J Agric Res 47(3):351–361

    Article  CAS  Google Scholar 

  • Di HJ, Cameron KC (2006) Nitrous oxide emissions from two dairy pasture soils as affected by different rates of a fine particle suspension nitrification inhibitor, dicyandiamide. Biol Fertil Soils 42:472–480

    Article  CAS  Google Scholar 

  • Di HJ, Cameron KC (2016) Inhibition of nitrification to mitigate nitrate leaching and nitrous oxide emissions in grazed grassland: a review. J Soils Sediments 16:1401–1420

    Article  CAS  Google Scholar 

  • Di HJ, Cameron KC, Sherlock RR (2007) Comparison of the effectiveness of a nitrification inhibitor, dicyandiamide, in reducing nitrous oxide emissions in four different soils under different climatic and management conditions. Soil Use Manag 23:1–9

    Article  Google Scholar 

  • Di HJ, Cameron KC, Shen JP, Winefield C, O’Callaghan M, Bowatte S, He J (2009) Nitrification driven by bacteria and not archaea in nitrogen-rich grassland soils. Nat Geosci 2:621–624

    Article  CAS  Google Scholar 

  • Di HJ, Cameron KC, Shen J-P, Winefield CS, O'Callaghan M, Bowatte S, He J-Z (2010) Ammonia-oxidizing bacteria and archaea grow under contrasting soil nitrogen conditions. FEMS Microbiol Ecol 72:386–394

    Article  CAS  Google Scholar 

  • Di HJ, Cameron KC, Podolyan A, Robinson A (2014) Effectof soil moisture status and a nitrification inhibitor, dicyandiamide, on ammonia oxidizer and denitrifier growth and nitrous oxide emissions in a grassland soil. Soil Biol Biochem 73:59–68

    Article  CAS  Google Scholar 

  • Di HJ, Cameron KC, Podolyan A, Edwards GR, de Klein CA, Dynes R, Woods R (2016) The potential of using alternative pastures, forage crops and gibberellic acid to mitigate nitrous oxide emissions. J Soils Sediments 16:2252–2262

    Article  CAS  Google Scholar 

  • Dietz M, Machill S, Hoffmann HC, Schmidtke K (2013) Inhibitory effects of Plantago lanceolata L. on soil N mineralization. Plant Soil 368:445–458

    Article  CAS  Google Scholar 

  • Edwards G, De Ruiter J, Dalley D, Pinxterhuis J, Cameron K, Bryant R, Di H, Malcolm B, Chapman D (2014) Urinary nitrogen concentration of cows grazing fodder beet, kale and kale-oat forage systems in winter, Proceedings of the 5th Australasian Dairy Science Symposium, pp 144–147

  • Firestone MK, Davidson EA (1989) Microbiological basis of NO and N2O production and consumption in soils. In: Andreae MO, Schimel DS (eds) Exchange of trace gases between terrestrial ecosystems and the atmosphere. John Wiley & Sons, Chichester, pp 7–21

    Google Scholar 

  • Francis CA, Roberts KJ, Beman JM, Santoro AE, Oakley BB (2005) Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proc Natl Acad Sci U S A 102:14683–14688

    Article  CAS  Google Scholar 

  • Gardiner C, Clough T, Cameron K, Di H, Edwards G, de Klein C (2016) Potential for forage diet manipulation in New Zealand pasture ecosystems to mitigate ruminant urine derived N2O emissions: a review. New Zeal J Agr Res 59:301–317

    Article  CAS  Google Scholar 

  • Hallin S, Lindgren P-E (1999) PCR detection of genes encoding nitrite reductase in denitrifying bacteria. Appl Environ Microbiol 65:1652–1657

    CAS  Google Scholar 

  • He JZ, Hu HW, Zhang LM (2012) Current insights into the autotrophic thaumarchaeal ammonia oxidation in acidic soils. Soil Biol Biochem 55:146–154

    Article  CAS  Google Scholar 

  • Hu HW, Chen D, He JZ (2015) Microbial regulation of terrestrial nitrous oxide formation: understanding the biological pathways for prediction of emission rates. FEMS Microbiol Rev 39(5):729–749

    Article  CAS  Google Scholar 

  • Hutchinson G, Mosier A (1981) Improved soil cover method for field measurement of nitrous oxide fluxes. Soil Sci Soc Am J 45:311–316

    Article  CAS  Google Scholar 

  • Jarvis SC, Schofield D, Pain B (1995) Nitrogen cycling in grazing systems. In: Bacon PE (ed) Nitrogen fertilization and the environment. Marcel Dekker, New York, pp 381–419

  • Jones CM, Graf DR, Bru D, Philippot L, Hallin S (2013) The unaccounted yet abundant nitrous oxide-reducing microbial community: a potential nitrous oxide sink. ISME J 7:417–426

    Article  CAS  Google Scholar 

  • Kloos K, Mergel A, Rösch C, Bothe H (2001) Denitrification within the genus Azospirillum and other associative bacteria. Funct Plant Biol 28:991–998

    Article  Google Scholar 

  • Li CY, Di HJ, Cameron KC, Podolyan A, Zhu BCH (2016) Effect of different land use and land use change on ammonia oxidiser abundance and N2O emissions. Soil Biol Biochem 96:169–175

    Article  CAS  Google Scholar 

  • Luo J, Ledgard S, Lindsey S (2008) A test of a winter farm management option for mitigating nitrous oxide emissions from a dairy farm. Soil Use Manag 24:121–130

    Article  Google Scholar 

  • Luo J, Sun XZ, Pacheco D, Ledgard S, Lindsey S, Hoogendoorn CJ, Wise B, Watkins N (2015) Nitrous oxide emission factors for urine and dung from sheep fed either fresh forage rape (Brassica napus L.) or fresh perennial ryegrass (Lolium perenne L.) Animal 9:534–543

    Article  CAS  Google Scholar 

  • McCarty G, Bremner J, Schmidt E (1991) Effects of phenolic acids on ammonia oxidation by terrestrial autotrophic nitrifying microorganisms. FEMS Microbiol Lett 85:345–349

    Article  CAS  Google Scholar 

  • MFE (2015) New Zealand’s greenhouse gas inventory 1990–2013. Ministry for the Environment, Wellington

    Google Scholar 

  • Michotey V, Méjean V, Bonin P (2000) Comparison of methods for quantification of cytochrome cd 1-denitrifying bacteria in environmental marine samples. Appl Environ Microbiol 66:1564–1571

    Article  CAS  Google Scholar 

  • Mosier A, Kroeze C, Nevison C, Oenema O, Seitzinger S, van Cleemput O (1998) Closing the global N2O budget: nitrous oxide emissions through the agricultural nitrogen cycle—OECD/IPCC/IEA phase II development of IPCC guidelines for national greenhouse gas inventory methodology. Nutr Cycl Agroecosys 52:225–248

    Article  CAS  Google Scholar 

  • Rice EL, Pancholy SK (1974) Inhibition of nitrification by climax ecosystems. III. Inhibitors other than tannins. Am J Bot 61:1095–1103

    Article  CAS  Google Scholar 

  • Rotthauwe J-H, Witzel K-P, Liesack W (1997) The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl Environ Microbiol 63:4704–4712

    CAS  Google Scholar 

  • Selbie D, Cameron K, Di H, Moir J, Lanigan G, Richards K (2014) The effect of urinary nitrogen loading rate and a nitrification inhibitor on nitrous oxide emissions from a temperate grassland soil. J Agr Sci 152:159–171

    Article  Google Scholar 

  • Throbäck IN, Enwall K, Jarvis Å, Hallin S (2004) Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE. FEMS Microbiol Ecol 49:401–417

    Article  Google Scholar 

  • Totty V, Greenwood S, Bryant R, Edwards G (2013) Nitrogen partitioning and milk production of dairy cows grazing simple and diverse pastures. J Dairy Sci 96:141–149

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We would like to thank the New Zealand Agricultural Greenhouse Gas Research Centre (NZAGRC), The Catalyst Fund (Seeding) and CSC (CSC No.201503270015) for funding and Steve Moore, Carole Barlow, Jie Lei, Qian Liang and Barry Anderson of Lincoln University for the technical support.

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Correspondence to Hong Jie Di.

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Responsible editor: Weixin Ding

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Yao, B., Di, H.J., Cameron, K.C. et al. Understanding the mechanisms for the lower nitrous oxide emissions from fodder beet urine compared with kale urine from dairy cows. J Soils Sediments 18, 85–93 (2018). https://doi.org/10.1007/s11368-017-1780-7

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  • DOI: https://doi.org/10.1007/s11368-017-1780-7

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