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Immunoradiometric assay for in-vitro determination of prolactin hormone in human serum or plasma using solid phase anti-PRL cellulose particles

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Abstract

The objective of the present study was oriented to produce and purify polyclonal anti-PRL antibody as the main key store in immunoradiometric assay (IRMA) using solid phase cellulose particles for determination of PRL in human sera. The preparation of 125I-PRL was carried out by lactoperoxidase method for estimation of the titre of antibody production. The preparation of standards was undertaken. The activation of cellulose particles using 1,1-carbonyl diimidazole (CDI) and coupling of these solid phase particles with purified Rabbit anti-PRL were carried out. Optimization and validation of the assay were carried out. Results revealed that the produced PRL polyclonal antibodies have high titre. Cellulose particles IRMA system was highly sensitive and specific. The intra- and inter-assay variations were satisfactory. The recovery and dilution tests indicated accurate calibration and appropriate matrix. The present technique agreed well with IRMA commercial kit. These cellulose particles retained their characteristics during storage for 6 months at 4 °C. In conclusion, this low cost assay could be used as a useful diagnostic tool for diagnosis and follow up of galactorrhea, infertility and pituitary adenoma.

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  1. Labeled Compound Department, Hot Laboratories Center, Atomic Energy Authority, P.O. Box 13759, Cairo, Egypt

    H. M. Shafik

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  1. H. M. Shafik
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Shafik, H.M. Immunoradiometric assay for in-vitro determination of prolactin hormone in human serum or plasma using solid phase anti-PRL cellulose particles. J Radioanal Nucl Chem 281, 639–646 (2009). https://doi.org/10.1007/s10967-009-0043-5

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  • DOI: https://doi.org/10.1007/s10967-009-0043-5

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