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Constituents of leaves from Bauhinia curvula Benth. exert gastroprotective activity in rodents: role of quercitrin and kaempferol

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Abstract

The Bauhinia genus is known as “Pata-de-Vaca” and a wide variety of these species are used in Brazilian folk medicine due to their gastroprotective properties. This study aimed to investigate the antiulcer efficacy of the hydroalcoholic extract from B. curvula (HEBC) leaves, as well as its semi-purified fraction (SPFr) and the contribution of their phytochemicals constituents for this effect. For that, ethanol 60%/HCl 0.3 M- and indomethacin-induced gastric ulcer were performed in rodents. Gastric ulcerated tissues were processed for histological, histochemical and biochemical analysis. The oral treatment with HEBC and SPFr decreased the gastric ulcer induced by ethanol/HCl in mice and by indomethacin (only HEBC) in rats. The gastroprotective effect of HEBC was abolished in mice pretreated with Nω-Nitro-l-arginine methyl ester, N-Ethylmaleimide, glibenclamide or indomethacin. Both HEBC and SPFr reduced myeloperoxidase activity in parallel with a decrease of lipoperoxides content at the site of the lesion. On the other hand, HEBC did not alter volume, pH, total acidity or pepsin activity of acid gastric secretion in rats, and neither inhibited the in vitro H(+),K(+)-ATPase activity. Additionally, the compounds identified and isolated from the SPFr, the flavonoids quercitrin (65%) and kaempferol (35%), were able to diminish the extent of ulcerated area induced by both ethanol/HCl and indomethacin. Taking together, these findings show that B. curvula extracts present gastroprotective effect, mainly explained by the presence of flavonoids quercitrin and kaempferol, which may possibly improve the defensive factors of gastric mucosa.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí-UNIVALI, Rua Uruguai, 458, 88302-901, Itajaí, SC, Brazil

    Ana Paula Beber, Priscila de Souza, Thaise Boeing, Lincon Bordignon Somensi, Luísa Nathália Bolda Mariano, Benhur Judah Cury, Sérgio Faloni de Andrade & Luísa Mota da Silva

  2. Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Estadual do Paraná, Curitiba, Brazil

    Ligia Moura Burci & Cristiane Bezerra da Silva

  3. Programa de Pós-Graduação em Química, Universidade Estadual do Mato Grosso do Sul, Campo Grande, Brazil

    Euclésio Simionatto

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  1. Ana Paula Beber
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  2. Priscila de Souza
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Correspondence to Priscila de Souza.

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Beber, A.P., de Souza, P., Boeing, T. et al. Constituents of leaves from Bauhinia curvula Benth. exert gastroprotective activity in rodents: role of quercitrin and kaempferol. Inflammopharmacol 26, 539–550 (2018). https://doi.org/10.1007/s10787-017-0313-8

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  • DOI: https://doi.org/10.1007/s10787-017-0313-8

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